Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gao, B.
Right arrow Articles by Meier, P. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gao, B.
Right arrow Articles by Meier, P. J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 47, 1255-1264, October 1999, Copyright © 1999, The Histochemical Society, Inc.

ARTICLE

Localization of the Organic Anion Transporting Polypeptide 2 (Oatp2) in Capillary Endothelium and Choroid Plexus Epithelium of Rat Brain

Bo Gaoa, Bruno Stiegera, Birgitta Noéa, Jean-Marc Fritschya, and Peter J. Meiera
a Division of Clinical Pharmacology and Toxicology, Department of Medicine, University Hospital, and Institute of Pharmacology, University of Zurich, Zurich, Switzerland

Correspondence to: Peter J. Meier, Div. of Clinical Pharmacology and Toxicology, Dept. of Medicine, University Hospital, CH-8091 Zurich, Switzerland.

In this study we investigated the distribution of a recently cloned polyspecific organic anion transporting polypeptide (Oatp2) in rat brain by nonradioactive in situ hybridization histochemistry and immunofluorescence microscopy. The results demonstrate that Oatp2 is expressed in brain capillary and in plexus epithelial cells. At the blood–brain barrier (BBB), Oatp2 expression could be co-localized with the endothelial marker vWF (von Willebrand factor) but not with the astrocyte marker GFAP (glial fibrillary acidic protein). In choroid plexus epithelial cells, Oatp2 could be localized to the basolateral cell pole, whereas the first member of the Oatp gene family of membrane transporters to be cloned (Oatp1) co-localized with the {alpha}1-subunit of Na,K-ATPase at the apical plasma membrane domain. Because Oatp1 and Oatp2 have been previously shown to mediate transmembrane transport of a wide variety of amphipathic organic compounds, including many drugs and other xenobiotics, the histochemical localization of Oatp2 at the BBB and of Oatp1 and Oatp2 in the choroid plexus imply a role for these transporters in the active exchange of amphipathic solutes between the blood, brain, and cerebrospinal fluid compartments. (J Histochem Cytochem 47:1255–1263, 1999)

Key Words: drug transport, organic anion transporter, blood–brain barrier, choroid plexus


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 BrainHome page
E. Kilic, A. Spudich, U. Kilic, K. M. Rentsch, R. Vig, C. M. Matter, H. Wunderli-Allenspach, J.-M. Fritschy, C. L. Bassetti, and D. M. Hermann
ABCC1: a gateway for pharmacological compounds to the ischaemic brain
Brain, October 1, 2008; 131(10): 2679 - 2689.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
D. S. Miller, B. Bauer, and A. M. S. Hartz
Modulation of P-Glycoprotein at the Blood-Brain Barrier: Opportunities to Improve Central Nervous System Pharmacotherapy
Pharmacol. Rev., June 1, 2008; 60(2): 196 - 209.
[Abstract] [Full Text] [PDF]

Home page
 Drug Metab. Dispos.Home page
B. M. Johnson, P. Zhang, J. D. Schuetz, and K. L. R. Brouwer
CHARACTERIZATION OF TRANSPORT PROTEIN EXPRESSION IN MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN (MRP) 2-DEFICIENT RATS
Drug Metab. Dispos., April 1, 2006; 34(4): 556 - 562.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
B. Kis, T. Isse, J. A. Snipes, L. Chen, H. Yamashita, Y. Ueta, and D. W. Busija
Effects of LPS stimulation on the expression of prostaglandin carriers in the cells of the blood-brain and blood-cerebrospinal fluid barriers
J Appl Physiol, April 1, 2006; 100(4): 1392 - 1399.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
B. Bauer, A. M. S. Hartz, G. Fricker, and D. S. Miller
Modulation of p-Glycoprotein Transport Function at the Blood-Brain Barrier
Experimental Biology and Medicine, February 1, 2005; 230(2): 118 - 127.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
Y.-J. Lee, H. Kusuhara, J. W. Jonker, A. H. Schinkel, and Y. Sugiyama
Investigation of Efflux Transport of Dehydroepiandrosterone Sulfate and Mitoxantrone at the Mouse Blood-Brain Barrier: A Minor Role of Breast Cancer Resistance Protein
J. Pharmacol. Exp. Ther., January 1, 2005; 312(1): 44 - 52.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
R. Kikuchi, H. Kusuhara, T. Abe, H. Endou, and Y. Sugiyama
Involvement of Multiple Transporters in the Efflux of 3-Hydroxy-3-methylglutaryl-CoA Reductase Inhibitors across the Blood-Brain Barrier
J. Pharmacol. Exp. Ther., December 1, 2004; 311(3): 1147 - 1153.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
Y. Zhang, J. D. Schuetz, W. F. Elmquist, and D. W. Miller
Plasma Membrane Localization of Multidrug Resistance-Associated Protein Homologs in Brain Capillary Endothelial Cells
J. Pharmacol. Exp. Ther., November 1, 2004; 311(2): 449 - 455.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
C. M. Breen, D. B. Sykes, C. Baehr, G. Fricker, and D. S. Miller
Fluorescein-methotrexate transport in rat choroid plexus analyzed using confocal microscopy
Am J Physiol Renal Physiol, September 1, 2004; 287(3): F562 - F569.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. Sykes, D. H. Sweet, S. Lowes, S. K. Nigam, J. B. Pritchard, and D. S. Miller
Organic anion transport in choroid plexus from wild-type and organic anion transporter 3 (Slc22a8)-null mice
Am J Physiol Renal Physiol, May 1, 2004; 286(5): F972 - F978.
[Abstract] [Full Text] [PDF]

Home page
 Drug Metab. Dispos.Home page
S. Choudhuri, N. J. Cherrington, N. Li, and C. D. Klaassen
CONSTITUTIVE EXPRESSION OF VARIOUS XENOBIOTIC AND ENDOBIOTIC TRANSPORTER mRNAs IN THE CHOROID PLEXUS OF RATS
Drug Metab. Dispos., November 1, 2003; 31(11): 1337 - 1345.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Sugiyama, H. Kusuhara, H. Taniguchi, S. Ishikawa, Y. Nozaki, H. Aburatani, and Y. Sugiyama
Functional Characterization of Rat Brain-specific Organic Anion Transporter (Oatp14) at the Blood-Brain Barrier: HIGH AFFINITY TRANSPORTER FOR THYROXINE
J. Biol. Chem., October 31, 2003; 278(44): 43489 - 43495.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
J. E. Gibbs, T. Rashid, and S. A. Thomas
Effect of Transport Inhibitors and Additional Anti-HIV Drugs on the Movement of Lamivudine (3TC) across the Guinea Pig Brain Barriers
J. Pharmacol. Exp. Ther., September 1, 2003; 306(3): 1035 - 1041.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
N. Mizuno, T. Niwa, Y. Yotsumoto, and Y. Sugiyama
Impact of Drug Transporter Studies on Drug Discovery and Development
Pharmacol. Rev., September 1, 2003; 55(3): 425 - 461.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
R. Kikuchi, H. Kusuhara, D. Sugiyama, and Y. Sugiyama
Contribution of Organic Anion Transporter 3 (Slc22a8) to the Elimination of p-Aminohippuric Acid and Benzylpenicillin across the Blood-Brain Barrier
J. Pharmacol. Exp. Ther., July 1, 2003; 306(1): 51 - 58.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
T. Suzuki, T. Onogawa, N. Asano, H. Mizutamari, T. Mikkaichi, M. Tanemoto, M. Abe, F. Satoh, M. Unno, K. Nunoki, et al.
Identification and Characterization of Novel Rat and Human Gonad-Specific Organic Anion Transporters
Mol. Endocrinol., July 1, 2003; 17(7): 1203 - 1215.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. Trauner and J. L. Boyer
Bile Salt Transporters: Molecular Characterization, Function, and Regulation
Physiol Rev, April 1, 2003; 83(2): 633 - 671.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Interv.Home page
W. M. Pardridge
BLOOD-BRAIN BARRIER DRUG TARGETING: THE FUTURE OF BRAIN DRUG DEVELOPMENT
Mol. Interv., March 1, 2003; 3(2): 90 - 105.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
S. Ohtsuki, T. Takizawa, H. Takanaga, N. Terasaki, T. Kitazawa, M. Sasaki, T. Abe, K.-i. Hosoya, and T. Terasaki
In Vitro Study of the Functional Expression of Organic Anion Transporting Polypeptide 3 at Rat Choroid Plexus Epithelial Cells and Its Involvement in the Cerebrospinal Fluid-to-Blood Transport of Estrone-3-Sulfate
Mol. Pharmacol., March 1, 2003; 63(3): 532 - 537.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
B. Hagenbuch, B. Gao, and Peter. J. Meier
Transport of Xenobiotics Across the Blood-Brain Barrier
Physiology, December 1, 2002; 17(6): 231 - 234.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. H. Sweet, D. S. Miller, J. B. Pritchard, Y. Fujiwara, D. R. Beier, and S. K. Nigam
Impaired Organic Anion Transport in Kidney and Choroid Plexus of Organic Anion Transporter 3 (Oat3 (Slc22a8)) Knockout Mice
J. Biol. Chem., July 19, 2002; 277(30): 26934 - 26943.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
Y. Nagata, H. Kusuhara, H. Endou, and Y. Sugiyama
Expression and Functional Characterization of Rat Organic Anion Transporter 3 (rOat3) in the Choroid Plexus
Mol. Pharmacol., May 1, 2002; 61(5): 982 - 988.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
G. L. Guo, J. Staudinger, K. Ogura, and C. D. Klaassen
Induction of Rat Organic Anion Transporting Polypeptide 2 by Pregnenolone-16alpha -carbonitrile Is via Interaction with Pregnane X Receptor
Mol. Pharmacol., April 1, 2002; 61(4): 832 - 839.
[Abstract] [Full Text] [PDF]

Home page
 Drug Metab. Dispos.Home page
G. L. Guo, D. R. Johnson, and C. D. Klaassen
Postnatal Expression and Induction by Pregnenolone-16alpha -Carbonitrile of the Organic Anion-Transporting Polypeptide 2 in Rat Liver
Drug Metab. Dispos., March 1, 2002; 30(3): 283 - 288.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
A. Ito, K. Yamaguchi, T. Onogawa, M. Unno, T. Suzuki, T. Nishio, T. Suzuki, H. Sasano, T. Abe, and M. Tamai
Distribution of Organic Anion-Transporting Polypeptide 2 (oatp2) and oatp3 in the Rat Retina
Invest. Ophthalmol. Vis. Sci., March 1, 2002; 43(3): 858 - 863.
[Abstract] [Full Text] [PDF]

Home page
 Drug Metab. Dispos.Home page
D. Sugiyama, H. Kusuhara, Y. Shitara, T. Abe, and Y. Sugiyama
Effect of 17beta -Estradiol-D-17beta -Glucuronide on the Rat Organic Anion Transporting Polypeptide 2-Mediated Transport Differs Depending on Substrates
Drug Metab. Dispos., February 1, 2002; 30(2): 220 - 223.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
B. Gao, A. Wenzel, C. Grimm, S. R. Vavricka, D. Benke, P. J. Meier, and C. E. Reme
Localization of Organic Anion Transport Protein 2 in the Apical Region of Rat Retinal Pigment Epithelium
Invest. Ophthalmol. Vis. Sci., February 1, 2002; 43(2): 510 - 514.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
G. L. Guo, S. Choudhuri, and C. D. Klaassen
Induction Profile of Rat Organic Anion Transporting Polypeptide 2 (oatp2) by Prototypical Drug-Metabolizing Enzyme Inducers That Activate Gene Expression through Ligand-Activated Transcription Factor Pathways
J. Pharmacol. Exp. Ther., January 1, 2002; 300(1): 206 - 212.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
G. Lee, S. Dallas, M. Hong, and R. Bendayan
Drug Transporters in the Central Nervous System: Brain Barriers and Brain Parenchyma Considerations
Pharmacol. Rev., December 1, 2001; 53(4): 569 - 596.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
D. Sugiyama, H. Kusuhara, Y. Shitara, T. Abe, P. J. Meier, T. Sekine, H. Endou, H. Suzuki, and Y. Sugiyama
Characterization of the Efflux Transport of 17beta -Estradiol-D-17beta -glucuronide from the Brain across the Blood-Brain Barrier
J. Pharmacol. Exp. Ther., July 1, 2001; 298(1): 316 - 322.
[Abstract] [Full Text]

Home page
 J. Histochem. Cytochem.Home page
J. F. Breininger and D. G. Baskin
Fluorescence In Situ Hybridization of Scarce Leptin Receptor mRNA using the Enzyme-Labeled Fluorescent Substrate Method and Tyramide Signal Amplification
J. Histochem. Cytochem., December 1, 2000; 48(12): 1593 - 1600.
[Abstract] [Full Text]

Home page
 Mol. Pharmacol.Home page
L. Li, P. J. Meier, and N. Ballatori
Oatp2 Mediates Bidirectional Organic Solute Transport: A Role for Intracellular Glutathione
Mol. Pharmacol., August 1, 2000; 58(2): 335 - 340.
[Abstract] [Full Text]

Home page
 J. Pharmacol. Exp. Ther.Home page
B. Gao, B. Hagenbuch, G. A. Kullak-Ublick, D. Benke, A. Aguzzi, and P. J. Meier
Organic Anion-Transporting Polypeptides Mediate Transport of Opioid Peptides across Blood-Brain Barrier
J. Pharmacol. Exp. Ther., July 1, 2000; 294(1): 73 - 79.
[Abstract] [Full Text]

Home page
 Drug Metab. Dispos.Home page
E. F. Choo, B. Leake, C. Wandel, H. Imamura, A. J. J. Wood, G. R. Wilkinson, and R. B. Kim
Pharmacological Inhibition of P-glycoprotein Transport Enhances the Distribution of HIV-1 Protease Inhibitors into Brain and Testes
Drug Metab. Dispos., June 1, 2000; 28(6): 655 - 660.
[Abstract] [Full Text]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1999