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Journal of Histochemistry and Cytochemistry, Vol. 47, 1525-1532, December 1999, Copyright © 1999, The Histochemical Society, Inc.

ARTICLE

Localization of Carbonic Anhydrase in Guinea Pig Bowman's Glands

Hiro-oki Okamuraa, Naonori Sugaia, and Kazunori Suzukia
a Department of Anatomy and Histology, Faculty of Medicine, Fukushima Medical University, Fukushima, Japan

Correspondence to: Hiro-oki Okamura, Dept. of Pathology and Laboratory Medicine, Medical U. of South Carolina, 165 Ashlet Avenue, Suite 309, PO Box 250908, Charleston, SC 29425.

We examined the histochemical localization of carbonic anhydrase (CA) in Bowman's glands by light and electron microscopy. Neither CAI nor CAII was detected immunohistochemically in the duct cells. However, by enzyme histochemistry the duct cells revealed electron-dense precipitates demonstrative of CA in the microvilli and intercellular digitations. The reaction product was also noted in small vesicles in the cytoplasm of duct cells. In cells of the acini, the well-developed short microvilli, basolateral cell membrane, and mitochondria along the basolateral membrane showed strong deposits indicating CA activity. Dense reaction product of CA was also detected in a small core within the electron-lucent granules of the secretory cells, although CAI and CAII were not detected by immunostaining in the secretory granules. Although the functional significance of CA in Bowman's glands is obscure, the enzyme may play a role in regulation of pH and ion balance in the mucous layer covering the olfactory epithelium. The presence of CA activity in the ducts suggests that these structures are not simple tubes serving as a conduit for secretory substances but participate in modifying the luminal content by secreting CA. (J Histochem Cytochem 47:1525–1531, 1999)

Key Words: carbonic anhydrase, immunohistochemistry, enzyme histochemistry, electron microscopy, Bowman's gland, guinea pig


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