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Journal of Histochemistry and Cytochemistry, Vol. 47, 919-928, July 1999, Copyright © 1999, The Histochemical Society, Inc.

ARTICLE

Multistratified Expression of Polysialic Acid and Its Relationship to VAChT-containing Neurons in the Inner Plexiform Layer of Adult Rat Retina

Akira Sawaguchia, Yoshiko Idatea, Soyuki Idea, Jun-ichi Kawanoa, Ryoko Nagaikea, Tsutomu Oinumaa, and Tatsuo Suganumaa
a Department of Anatomy, Miyazaki Medical College, Miyazaki, Japan

Correspondence to: Tatsuo Suganuma, Dept. of Anatomy, Miyazaki Medical College, 5200 Kihara, Kiyotake-cho, Miyazaki 889-1692, Japan.

We investigated the localization of polysialic acid (PSA), neural cell adhesion molecule (NCAM), and vesicular acetylcholine transporter (VAChT) in adult rat retina by using immunofluorescence with a confocal laser scanning microscope. Western blot analysis showed a typical broad smear of PSA and isoforms of NCAM (120, 140, and 180 kD). PSA immunofluorescence revealed multistratification in the inner plexiform layer (IPL). Dual immunostaining for PSA and NCAM exhibited the selective co-expression of PSA and NCAM on Müller cells. Moreover, dual immunolabeling for PSA and VAChT completely separated the five strata in the IPL. Strata 1, 3, and 5 were immunoreactive for PSA and Strata 2 and 4 for VAChT. These results suggest the possibility that PSA molecules on Müller cells are spatially related to ON and OFF retinal channels in the IPL. (J Histochem Cytochem 47:919–927, 1999)

Key Words: polysialic acid, neural cell adhesion molecule, vesicular acetylcholine, transporter, rat, retina, Müller cell, cholinergic neuron, confocal laser scanning, microscopy


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