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Journal of Histochemistry and Cytochemistry, Vol. 49, 19-28, January 2001, Copyright © 2001, The Histochemical Society, Inc.

ARTICLE

Lectin and Proteoglycan Histochemistry of Feline Pacinian Corpuscles

Klaus Samesa, Zdenek Halataa, Milan Jojovica, Els J. M. van Dammeb, Willy J. Peumansb, Bertrand Delpechc, Brigitte Asmusa, and Udo Schumachera
a Institute for Anatomy, University Hospital Hamburg–Eppendorf, Hamburg, Germany
b Laboratorium voor Fytopathologie en Plantenbescherming, Katholieke Universiteit Leuven, Leuven Belgium
c Laboratoire d'Oncologie Moléculaire, Centre Henri-Becquerel, Rouen, France

Correspondence to: Klaus Sames, Inst. for Anatomy, University Hospital Hamburg–Eppendorf, Martinistr. 52, D-20246 Hamburg, Germany.

We studied carbohydrate residues of glycoproteins and proteoglycans (PGs) in peritoneal Pacinian corpuscles of five adult cats. Terminal monosaccharides of glycoproteins and related polysaccharides were identified by lectin histochemistry and the PGs and glycosaminoglycans (GAGs) by specific antibodies. The most intensive lectin staining reactions indicated an abundance of glycoconjugates with terminal mannose (Man) or sialic acid residues, but no complex-type oligosaccharides were detected within the corpuscles. Terminal fucose (Fuc) and galactose (Gal) residues typical for O-linked mucin-type glycoproteins generally associated with high water binding capacity were also absent. Antibodies against unsulfated chondroitin (C-0-S), chondroitin-4-sulfate (C-4-S), and decorin showed positive reactions in the interfibrillar spaces between the lamellae, around collagen fibers, and around the lamellae of the perineural capsule, especially in the outer parts known to contain Type II collagen. Biglycan showed a preference for the innermost part of the perineural capsule (intermediate layer), known to contain Type V collagen. Collagen V and biglycan are both linked to growth processes. Hyaluronic acid (HA), chondroitin-6-sulfate (C-6-S) chains, and a chondroitin sulfate proteoglycan (CSPG) were co-localized in the terminal glia. The study of carbohydrates with high water binding capacity may contribute to our understanding of the high viscoelasticity of Pacinian corpuscles.

(J Histochem Cytochem 49:19–28, 2001)

Key Words: carbohydrate histochemistry, hyaluronic acid, lectins, Pacinian corpuscles, proteoglycans


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