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Journal of Histochemistry and Cytochemistry, Vol. 49, 41-48, January 2001, Copyright © 2001, The Histochemical Society, Inc.

ARTICLE

Expression of the ß6 Integrin Subunit Is Associated with Sites of Neutrophil Influx in Lung Epithelium

Lisa A. Millera, Nancy L. Barnetta, Dean Sheppardb, and Dallas M. Hydea
a Department of Anatomy, Physiology, and Cell Biology, School of Veterinary Medicine, and Center for Comparative Respiratory Biology and Medicine, University of California, Davis, California
b Lung Biology Center, University of California, San Francisco, California

Correspondence to: Lisa A. Miller, Dept. of Anatomy, Physiology, and Cell Biology, School of Veterinary Medicine, U. of California, Davis, Davis, CA 95616. E-mail: lmiller@ucdavis.edu

Inhalation of ozone by Rhesus monkeys results in epithelial injury and granulocyte influx in both conducting airways and respiratory bronchioles. We have reported that ozone-induced neutrophil recruitment and subsequent epithelial repair can be inhibited in vivo with a CD18 antibody. The antibody-mediated effect is abrogated by local instillation of C5a (a CD18-independent neutrophil chemoattractant), thereby demonstrating a role for neutrophils in lung epithelial repair processes. As an extension of this study, we examined the effect of ozone and neutrophil influx on epithelial expression of the ß6 integrin, an adhesion molecule associated with proliferation and repair. Expression of ß6 integrin was determined by immunohistochemistry for ozone-exposed monkeys treated with either control immunoglobulins or a CD18 antibody. The tracheal epithelium of ozone-exposed monkeys treated with control immunglobulins expressed the ß6 integrin. In contrast, the tracheal epithelium of ozone-exposed monkeys treated with CD18 antibody exhibited very low to undetectable expression of ß6 integrin. In association with C5a instillation and neutrophil influx, ß6 integrin was also observed in respiratory bronchiolar epithelium from both control and ozone-exposed animals. These findings cumulatively suggest that lung epithelial cell expression of ß6 integrin is associated with sites of neutrophil recruitment. (J Histochem Cytochem 49:41–47, 2001)

Key Words: neutrophil, lung, epithelium, integrin


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