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Journal of Histochemistry and Cytochemistry, Vol. 49, 9-18, January 2001, Copyright © 2001, The Histochemical Society, Inc.

ARTICLE

Cell-specific Processing of Chromogranin A in Endocrine Cells of the Rat Stomach

Per Norléna, William J. Curryb, Maria Björkqvista, Aron Mauleb, Rodat T. Cunninghamb, Robert B. Hoggb, Pat Harriottc, Colin F. Johnstonb, John C. Huttond, and Rolf Håkansona
a Department of Pharmacology, Institute of Physiological Sciences, University of Lund, Sweden
b Department of Medicine, The Queen's University of Belfast, Belfast, North Ireland
c School of Biology and Biochemistry, The Queen's University of Belfast, Belfast, North Ireland
d Barbara Davis Center for Childhood Diabetes, University of Colorado Health Sciences Center, Boulder, Colorado

Correspondence to: Rolf Håkanson, Dept. of Pharmacology, University of Lund, Sölvegatan 10, S-223 62 Lund, Sweden. E-mail: Rolf.Hakanson@farm.lu.se

The rat stomach is rich in endocrine cells. The acid-producing (oxyntic) mucosa contains ECL cells, A-like cells, and somatostatin (D) cells, and the antrum harbours gastrin (G) cells, enterochromaffin (EC) cells and D cells. Although chromogranin A (CgA) occurs in all these cells, its processing appears to differ from one cell type to another. Eleven antisera generated to different regions of rat CgA, two antisera generated to a human (h) CgA sequences, and one to a bovine (b) CgA sequence, respectively, were employed together with antisera directed towards cell-specific markers such as gastrin (G cells), serotonin (EC cells), histidine decarboxylase (ECL cells) and somatostatin (D cells) to characterize the expression of CgA and CgA-derived peptides in the various endocrine cell populations of the rat stomach. In the oxyntic mucosa, antisera raised against CgA291–319 and CGA316–321 immunostained D cells exclusively, whereas antisera raised against bCgA82–91 and CgA121–128 immunostained A-like cells and D cells. Antisera raised against CgA318–349 and CgA437–448 immunostained ECL cells and A-like cells, but not D cells. In the antrum, antisera against CgA291–319 immunostained D cells, and antisera against CgA351–356 immunostained G cells. Our observations suggest that each individual endocrine cell type in the rat stomach generates a unique mixture of CgA-derived peptides, probably reflecting cell-specific differences in the post-translational processing of CgA and its peptide products. A panel of antisera that recognize specific domains of CgA may help to identify individual endocrine cell populations. (J Histochem Cytochem 49:9–18, 2001)

Key Words: chromogranin A, ECL cell, D cell, A-like cell, EC cell, G cell, processing


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