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Journal of Histochemistry and Cytochemistry, Vol. 49, 1199-1204, October 2001, Copyright © 2001, The Histochemical Society, Inc.

ARTICLE

Changes in the Distribution of Anionic Constituents in Secretory Granules of Mouse Pancreatic Acinar Cells After Pilocarpine-induced Degranulation

Tanya Shoichetmana, Ehud Skutelskya, Sylvia Lewa, and Ilan Hammela
a Department of Pathology, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, Israel

Correspondence to: Ilan Hammel, Dept. of Pathology, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, Israel. E-mail: ilanh@patholog.tau.ac.il

We used cationized colloidal gold (CCG) to investigate the distribution of anionic sites in different secretory granules of mouse pancreatic acinar cell regranulation. Localization of anionic sites with CCG was carried out on ultrathin sections of a mouse pancreas, fixed in Karnovsky's fixative and OsO4 and embedded in Araldite. After pilocarpine-stimulated degranulation, there was a marked diminution in the anionic charge density of immature and mature granules of the 4-hr group ({approx}43.0 gold particles/µm2) compared to the 8-hr mature granules group ({approx}64.6 gold particles/µm2). Scattergram analysis to investigate the correlation between section profile size and cationized gold labeling density revealed a reverse correlation, the small granule profiles demonstrated a higher density compared to the larger profiles of the same group. On the basis of these observations, it appears that a post-translational processing of secretory content influences the granule anionic charge and thus may affect the intragranular buffer capacity.

(J Histochem Cytochem 49:1199–1204, 2001)

Key Words: pancreas, secretory granules, polyanions, cationic gold, quantitative cytochemistry


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