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Journal of Histochemistry and Cytochemistry, Vol. 49, 1261-1268, October 2001, Copyright © 2001, The Histochemical Society, Inc.

ARTICLE

A Comparative Quantitative Analysis of Laminin-5 in the Basement Membrane of Normal, Hyperplastic, and Malignant Oral Mucosa by Confocal Immunofluorescence Imaging

K. Michael Haasa,b, Alexander Berndta, Klaus J. Stillera, Peter Hyckelb, and Hartwig Kosmehla
a Institute of Pathology, Friedrich Schiller University, Jena, Germany
b Clinic of Maxillofacial Surgery, Friedrich Schiller University, Jena, Germany

Correspondence to: Hartwig Kosmehl, Institute of Pathology, Friedrich Schiller University, D-07740 Jena, Germany. E-mail: kosmehl@bach.med.uni-jena.de

Laminin-5 (Ln-5) is a heterotrimeric basement membrane (BM) molecule ({alpha}3ß3{gamma}2). It is a principal protein constituent of the anchoring filaments, which connect the BM with the hemidesmosomes of the basal keratinocytes and possess a crucial function in keratinocyte adhesion. Confocal immunofluorescence imaging is introduced for a quantitative evaluation of the Ln-5 content in the BM of oral squamous epithelium. The BM of normal oral mucosa was used as a reference (100%) for comparative analysis and showed a nearly uniform Ln-5 immunofluorescence intensity (99–100%). In all hyperplastic lesions of oral mucosa, the Ln-5 immunofluorescence intensity was increased (107–141%). The increased Ln-5 content in the BM of hyperplastic lesions suggests an increased keratinocyte–BM adhesion, possibly resulting in a higher stability of the oral mucosa. In contrast, in the oral squamous cell carcinoma (OSCC) invasive front, the remaining BM segments were characterized by a decrease in Ln-5 immunofluorescence intensity (35–74%). A stronger decrease of Ln-5-linked kerationocyte-BM adhesion correlates with a higher tumor grade. Because in central areas of carcinoma BM segments with a normal Ln-5 content could be demonstrated, the fundamental Ln-5 diminution in BM segments of the invasive front should be considered as an invasion-associated phenomenon.

(J Histochem Cytochem 49:1261–1268, 2001)

Key Words: laminin-5, invasion, squamous cell carcinoma, confocal laser scanning microscopy


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