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Journal of Histochemistry and Cytochemistry, Vol. 49, 1321-1322, October 2001, Copyright © 2001, The Histochemical Society, Inc.

BRIEF REPORT

Dual Y-chromosome Painting and Immunofluorescence Staining of Archival Human Liver Transplant Biopsies

S. R. Dundasa, S. Boylec, C. O. Bellamya, W. Hawkinsa, O. J. Gardena,b, J. A. Rossa, and W. Bickmorec
a Departments of Clinical and Surgical Sciences and Pathology, University of Edinburgh
b Scottish Liver Transplant Unit, Royal Infirmary Edinburgh
c MRC Human Genetics Unit, Western Infirmary, Edinburgh, Scotland

Correspondence to: S. R. Dundas, Dept. Clinical and Surgical Sciences, University of Edinburgh, Lister Place, Edinburgh SCO, EH3 9YW UK. E-mail: srdundas@yahoo.co.uk

Combining fluorescence in situ hybridization (FISH) and indirect immunofluorescence staining of protein markers provides a highly specific method for identifying chromosomes in phenotypically defined cells and tissues. We developed a technique enabling dual chromosome painting and immunofluorescence staining of archival formalin-fixed, paraffin-embedded material, and used this to phenotype chimeric cells in female-to-male human liver transplants. (J Histochem Cytochem 49:1321–1322, 2001)

Key Words: fluorescence in situ, hybridization, chromosome painting, Y-chromosome chimerism, liver allografts


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