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Journal of Histochemistry and Cytochemistry, Vol. 49, 1573-1580, December 2001, Copyright © 2001, The Histochemical Society, Inc.

ARTICLE

Immunohistological Analysis of Glutathione Transferase A4 Distribution in Several Human Tissues Using a Specific Polyclonal Antibody

Fabienne Desmotsa, Mary Rissela, Pascal Loyerb, Bruno Turlinb,c, and André Guillouzoa
a INSERM U456, Détoxication et Réparation Tissulaire, Faculté de Sciences Pharmaceutiques, Université de Rennes 1, France
b INSERM U522, Régulation des Equilibres Fonctionnels du Foie Normal et Pathologique, Hôpital de Pontchaillou, Rennes, France
c Laboratoire d'Anatomie Pathologique B, Hôpital Pontchaillou, Rennes, France

Correspondence to: André Guillouzo, INSERM U456, Faculté de Pharmacie, 2 Avenue du Pr. Léon Bernard, 35043 Rennes Cedex, France. E-mail: Andre.guillouzo@rennes.inserm.fr

We examined the cellular distribution of glutathione transferase A4 (GSTA4) in various human tissues by indirect immunoperoxidase using a specific polyclonal antibody raised in rabbit. This enzyme was localized in hepatocytes, bile duct cells, and vascular endothelial cells in liver, upper layers of keratinocytes and sebaceous and sweat glands in skin, proximal convoluted tubules in kidney, epithelial cells of mucosa and muscle cells in colon, muscle cells in heart, and neurons in brain. Staining was increased in pathological situations such as cirrhosis, UV-irradiated skin, and myocardial infarction and was strongly decreased in hepatocellular carcinoma. These results strongly support the view of a close correlation between cellular GSTA4 localization and the formation of reactive oxygen species in the tissues investigated. (J Histochem Cytochem 49:1573–1579, 2001)

Key Words: glutathione transferase A4, immunoperoxidase, human tissues, oxidative stress


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