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Journal of Histochemistry and Cytochemistry, Vol. 49, 271-278, February 2001, Copyright © 2001, The Histochemical Society, Inc.

ARTICLE

Epithelial Cell Differentiation Pathways in the Human Prostate: Identification of Intermediate Phenotypes by Keratin Expression

David L. Hudsona, Adam T. Guya, Patricia Frya, Michael J. O'Hareb, Fiona M. Wattc, and John R.W. Mastersa
a Institute of Urology and Nephrology, Research Laboratories, University College London Medical School,
b LICR/UCL Breast Cancer Laboratory, Department of Surgery, University College London Medical School
c Keratinocyte Laboratory, Imperial Cancer Research Fund, London, United Kingdom

Correspondence to: David L. Hudson, Inst. of Urology and Nephrology, Research Laboratories, University College London Medical School, 67-73 Riding House St., London WIW 7E7, UK. E-mail: d.hudson@ucl.ac.uk

The prostate grows slowly throughout adult life, leading to benign prostatic hyperplasia (BPH), which often results in urethral obstruction in later years. The symptoms of BPH are the second most common reason for surgery in men over 65. The aim of this study was to determine the relationship between cell proliferation and cell differentiation in BPH tissue. Using multiple antibodies, simultaneously detected with different fluorophore-conjugated secondary antibodies, several subpopulations of epithelial cells were detected. In addition to K14, basal cells also expressed keratins 15, 17, and 19 in various combinations, and some of the luminal cells also expressed K19 together with K8 and K18. Co-staining for cytokeratins and Ki-67 indicated that 44% of proliferative cells expressed K14 and 36% K19, although the difference was not statistically significant. This report provides a detailed description of the relationship between keratin expression and cell proliferation in the prostate and indicates that K19-positive cells form the link between the basal and luminal layers of the epithelium. (J Histochem Cytochem 49:271–278, 2001)

Key Words: prostate, epithelium, keratin, differentiation, stem cell, benign prostatic hyperplasia


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