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Journal of Histochemistry and Cytochemistry, Vol. 49, 777-782, June 2001, Copyright © 2001, The Histochemical Society, Inc.

ARTICLE

Cellular Localization of Group IIA Phospholipase A2 in Rats

Toshiyuki Yoshikawaa, Satoru Narusea, Motoji Kitagawaa, Hiroshi Ishiguroa, Masato Nagahamab, Etsuko Yasudab, Reiji Sembab, Minoru Tanakac, Kohji Nomurad, and Tetsuo Hayakawaa
a Department of Internal Medicine II, Nagoya University School of Medicine, Nagoya, Japan
b Departments of Anatomy II, Mie University School of Medicine, Tsu, Japan
c Biochemistry, Mie University School of Medicine, Tsu, Japan
d Shionogi Pharmaceutical Co., Ltd., Osaka, Japan

Correspondence to: Satoru Naruse, Department of Internal Medicine II, Nagoya U. School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8560, Japan. E-mail: snaruse@med.nagoya-u.ac.jp

It has been known that group II phospholipase A2 (PLA2) mRNA and protein are present in the homogenates of the spleen, lung, liver, and kidney in normal rats, but the cellular origin of this enzyme has not been yet identified. At present, five subtypes of group II PLA2 have been identified in mammals. Antibodies or mRNA probes previously used for detecting group II PLA2 need to be evaluated to identify the subtypes of group II PLA2. In this study we tried to identify group IIA PLA2-producing cells in normal rat tissues by in situ hybridization (ISH) using an almost full-length RNA probe for rat group IIA enzyme. Group IIA PLA2 mRNA was detected in megakaryocytes in the spleen and Paneth cells in the intestine by ISH. These cells were also immunopositive for an antibody raised against group IIA PLA2 isolated from rat platelets. Group IIA PLA2 mRNA-positive cells were not detected in lung, liver, kidney, and pancreas. Under normal conditions, group IIA PLA2-producing cells are splenic megakaryocytes and intestinal Paneth cells in rats.

(J Histochem Cytochem 49:777–782)

Key Words: group IIA phospholipase A2, immunohistochemistry, in situ hybridization, rat, spleen, megakaryocyte, intestine, Paneth cell


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