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Journal of Histochemistry and Cytochemistry, Vol. 49, 1003-1012, August 2001, Copyright © 2001, The Histochemical Society, Inc.

ARTICLE

Extensive DNA Fragmentation in Oxyphilic Cell Lesions of the Thyroid

Marco Volantea, Mauro Papottia, Patrizia Gugliottaa, Antonio Mighelib, and Gianni Bussolatia
a Departments of Biomedical Sciences and Oncology, University of Turin, Torino, Italy
b Neurosciences, University of Turin, Torino, Italy

Correspondence to: Marco Volante, Dept. of Biomedical Sciences and Oncology, University of Torino, via Santena 7, 10126 Torino, Italy. E-mail: marco.volante@molinette.unito.it

The in situ end-labeling (ISEL) method demonstrates DNA fragmentation, commonly regarded as a marker of apoptosis. We investigated by the ISEL procedure a series of 52 thyroid lesions, including 24 lesions of mitochondrion-rich oxyphilic cells, both benign and malignant, and 28 non-oxyphilic control tumors. A high percentage of nuclear ISEL staining (approximating to 100% in most cases) was observed in the vast majority of oxyphilic cells from both adenomas and carcinomas, in the absence of morphological apoptotic changes and with no immunocytochemical evidence of caspase activation. This pattern of DNA fragmentation was not observed in non-oxyphilic lesions and was confirmed in total extracted DNA. Moreover, a peculiar cytoplasmic staining was also observed in oxyphilic cells from both benign and malignant lesions, probably related to abnormal fragmentation of mitochondrial DNA. Similar staining patterns were detected in oxyphilic cell tumors of other organs (parathyroids, salivary glands, and kidneys). These findings are consistent with an extensive DNA fragmentation peculiar to oxyphilic cells, which is not directly related to apoptosis and whose origin and biological significance are presently unknown.

(J Histochem Cytochem 49:1003–1011, 2001)

Key Words: apoptosis, ISEL, oxyphilic tumors, thyroid


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