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Journal of Histochemistry and Cytochemistry, Vol. 49, 1057-1058, August 2001, Copyright © 2001, The Histochemical Society, Inc.

BRIEF REPORT

Monitoring Signal Transduction in Cancer: cDNA Microarray for Semiquantitative Analysis

H.-Ben Hsieha, Robert A. Lerscha, Daniel E. Callahana, Simon Haywardb, Mariwil Wongc, Orlo H. Clarkc, and Heinz-Ulrich G. Weiera
a Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, University of California–San Francisco, San Francisco, California
b Departments of Urology, University of California–San Francisco, San Francisco, California
c Surgery, University of California–San Francisco, San Francisco, California

Correspondence to: Robert A. Lersch, Life Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, MS 74-157, Berkeley, CA 94720. E-mail: ralersch@lbl.gov

This study targeted the development of a novel microarray tool to allow rapid determination of the expression levels of 58 different tyrosine kinase (tk) genes in small tumor samples. The goals were to define a reference probe for multi-sample comparison and to investigate the variability and reproducibility of the image acquisition and RT-PCR procedures. The small number of tk genes on our arrays enabled us to define a reference probe by artificially mixing all genes on the arrays. Such a probe provided contrast reference for comparative hybridization of control and sample DNA and enabled cross-comparison of more than two samples against one another. Comparison of signals generated from multiple scanning eliminated the concern of photo bleaching and scanner intrinsic noise. Tests performed with breast, thyroid, and prostate cancer samples yielded distinctive patterns and suggest the feasibility of our approach. Repeated experiments indicated reproducibility of such arrays. Up- or downregulated genes identified by this rapid screening are now being investigated with techniques such as in situ hybridization.

(J Histochem Cytochem 49:1057–1058, 2001)

Key Words: cDNA microarray, expression profiling, tyrosine kinase, reference probe


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