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HISTOCHEMICAL STUDY OF A FLUORIDE RESISTANT ACID PHOSPHATASE REACTION IN THE MOUSE DUODENUM

ROBERT C. BURT 1, BARBARA R. MEREDITH 1, and ROBERT C. GRAUER 1

1 William H. Singer Memorial Research Laboratory of the Allegheny General Hospital, Pittsburgh, Pennsylvania

A fluoride resistant acid phosphatase reaction (pH 5.0) has been demonstrated histochemically in the cuticular border of the mouse duodenum. This reaction was shown to be inactivated by the metal chelating agent, ethylene diamine tetraacetic acid, and reactivated by the following metallic salts : calcium chloride, cobalt(ous) nitrate, cupric sulfate, ferric chloride, ferric ammonium sulfate, ferrous sulfate, lead nitrate, lithium sulfate, magnesium sulfate, mercuric chloride, manganous chloride and zinc acetate. The two ferric salts produced the greatest amount of reactivation and cupric sulfate and manganous chloride produced the least amount of reactivation.

These characteristics of inactivation by a metal chelating agent and metal reactivation plus the fluoride resistance of the acid phosphatase reaction present in the cuticular border of the mouse duodenum are features more typical of an alkaline type of phosphatase rather than the acid variety. This suggests that alkaline type phosphatase may be demonstrable histochemically in the cuticular border of the mouse duodenum in the acid pH range.

Submitted on July 30, 1956


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