Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by LILLIE, R. D.
Right arrow Articles by GLENNER, G. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by LILLIE, R. D.
Right arrow Articles by GLENNER, G. G.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

HISTOCHEMICAL ALDEHYDE BLOCKADE BY ANILINE IN GLACIAL ACETIC ACID

R. D. LILLIE 1 and G. G. GLENNER 1

1 Laboratory of Pathology and Histochemistry, NIAMD, National Institutes of Health, Bethesda 14, Maryland

More rapid and more complete blockade of tissue aldehydes produced by hydrochloric, periodic or peracetic acid may be achieved by use of molar solutions of aniline in glacial acetic acid than by use of molar aniline hydrochloride in water. A solution of 1% benzidine or o-dianisidine in glacial acetic acid is more effective than the above aniline solution against aldehydes produced by peracetic acid from lipids, but less effective against aldehydes produced by periodic acid from carbohydrates.

Inclusion of 25 parts concentrated hydrochloric acid to 75 parts glacial acetic acid in the solvent impairs or inhibits the azomethine reaction of aniline with tissue aldehydes.

A 20 to 30 minute exposure to a 10% volume dilution of aniline in glacial acetic acid should be adequate for testing the aldehyde nature of Schiff reactive loci in tissue, and in most instances failure of a 3 hour treatment to impair or prevent the following Schiff reaction can be taken to exclude aldehyde.

The greater relative effect of o-dianisidine and benzidine in preventing the Schiff reaction of periodic or peracetic acid oxidized lipoids is of interest and merits further exploration.

The colors produced by the o-dianisidine condensation with tissue aldehydes are disappointingly pale and this reaction would not appear to have any great histologic value.

Submitted on September 11, 1956


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1957