Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by LILLIE, R. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by LILLIE, R. D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

ADAPTATION OF THE MOREL SISLEY PROTEIN DIAZOTIZATION PROCEDURE TO THE HISTOCHEMICAL DEMONSTRATION OF PROTEIN BOUND TYROSINE

R. D. LILLIE 1

1 Laboratory of Pathology and Histochemistry, NIAMD, National Institutes of Health, Bethesda 14, Maryland

Protein diazotization followed by alkaline coupling with a naphthol has been successfully applied for the histochemical localization of the protein bound tyrosine in tissues. Prolonged nitrous acid treatment in the cold is required (18-24 hours at 3°C). The best coupling reagent so far tried is 1-amimio-8-naphthol-4-sulfonic acid (S acid) and coupling succeeds best in a 1 % potassium hydroxide in 70 % alcohol, allowing 1 to 4 hours at 3°C. Topochemical results parallel those of the Millon reactions, with greater color intensities and superior histologic definition.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1957