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Journal of Histochemistry and Cytochemistry, Vol. 50, 1461-1468, November 2002, Copyright © 2002, The Histochemical Society, Inc.

ARTICLE

Expression of Hepatocyte Growth Factor mRNA in Rat Liver Cirrhosis Induced by N-nitrosodimethylamine as Evidenced by In Situ RT-PCR

Junko Hataa, Etsuko Ikedaa, Hiroshi Unoa, and Satoshi Asanoa
a Safety Research Department, Pharmaceuticals Development Research Laboratories, Teijin Limited, Tokyo, Japan

Correspondence to: Junko Hata, Safety Research Dept., Pharmaceuticals Development Research Labs, Teijin Ltd., 4-3-2 Asahigaoka, Hino, Tokyo 191-8512, Japan. E-mail: j.hata@teijin.co.jp

Hepatocyte growth factor (HGF) is a potent inducer of hepatocyte proliferation and is expressed during liver failure. In this study we used the in situ reverse transcriptase-polymerase chain reaction (RT-PCR) method to detect HGF mRNA expression in normal rat livers and cirrhotic rat livers induced by treatment with N-nitrosodimethylamine (DMN). In normal control livers, in situ RT-PCR detected HGF mRNA expression in Ito cells and Kupffer cells, both of which showed rounded morphologies. However, in the cirrhotic livers induced by DMN, HGF mRNA-positive cells were spindle-shaped and surrounded the hepatocytes located around the sinusoids. These cells appeared to be sinusoidal endothelial cells as well as Ito and Kupffer cells. Because it has been suggested that HGF expression is related to transforming growth factor-ß (TGF-ß) levels that may play an essential role in disease progression in cirrhotic livers, TGF-ß mRNA expression in normal and cirrhotic livers was also compared using in situ RT-PCR. Our results confirmed that expression of TGF-ß mRNA co-localized with HGF mRNA expression in the cirrhotic liver.

(J Histochem Cytochem 50:1461–1468, 2002)

Key Words: HGF, in situ RT-PCR, sinusoidal endothelial cells, Ito cells, Kupffer cells, TGF-ß


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