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Journal of Histochemistry and Cytochemistry, Vol. 50, 1475-1486, November 2002, Copyright © 2002, The Histochemical Society, Inc.

ARTICLE

Immunohistochemical Recognition of Human Follicular Dendritic Cells (FDCs) in Routinely Processed Paraffin Sections

Kunihiko Maedaa, Mikio Matsudab, Hitoshi Suzukia, and Hito-aki Saitoha
a Department of Pathology, Yamagata University School of Medicine, Yamagata, Japan
b Department of Nursing, School of Health Science, Yamagata Prefectural University of Health Science, Yamagata, Japan

Correspondence to: Kunihiko Maeda, Dept. of Pathology, Yamagata Univ. School of Medicine, 2-2-2 Iida-Nishi, Yamagata 990-9585, Japan. E-mail: kumaeda@med.id.yamagata-u.ac.jp

A number of monoclonal antibodies (MAbs) that recognize human follicular dendritic cells (FDCs) have been identified. Although some of them have already been applied individually in routine immunolabeling using formalin-fixed paraffin sections for diagnostic and experimental purposes, many antibodies are still employed only for immunolabeling using cryostat sections or particularly processed sections because they have been thought unsuitable for routine sections. A comprehensive examination re-evaluating their suitability in paraffin sections has not been reported. Accordingly, there is limited ability to examine the immunopathological contribution or diagnostic value of FDCs using routinely processed specimens or archived materials. In this study a broad panel of antibodies was systematically applied to the immunolabeling of paraffin sections of reactive tonsils or lymph nodes, in combination with advanced antigen retrieval (AR) techniques. Several antibodies, including Ki-M4p, X-11, 12B1, CNA.42, 1F8/BU32 (anti-CD21), BU38/1B12 (anti-CD23), Ber-MAC-DRC/To5 (anti-CD35), 1.4C3 (anti-CD106), NGFR5 (anti-nerve growth factor receptor p75), IIH6 (anti-CD55), 55K-2 (anti-fascin), and anti-S100 protein {alpha}-chain, were found to label FDCs in routine sections when combined with suitable AR techniques. Our results are easily adaptable for routine practice and provided useful suggestions concerning the immunopathological behavior and diversity of the particular cells.

(J Histochem Cytochem 50:1475–1485, 2002)

Key Words: formalin, immunohistochemistry, ABC method, unmasking, trypsin digestion, microwave irradiation, citrate buffer, EDTA


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