Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Portela–Gomes, G. M.
Right arrow Articles by Stridsberg, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Portela–Gomes, G. M.
Right arrow Articles by Stridsberg, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 50, 1487-1492, November 2002, Copyright © 2002, The Histochemical Society, Inc.

ARTICLE

Chromogranin A in the Human Gastrointestinal Tract: An Immunocytochemical Study with Region-specific Antibodies

Guida Maria Portela–Gomesa,c and Mats Stridsbergb
a Department of Genetics and Pathology, Unit of Pathology, University of Lisbon, Lisbon, Portugal
b Department of Medical Sciences, Clinical Chemistry, University of Lisbon, Lisbon, Portugal
c University Hospital, Uppsala, Sweden, and the Centres of Gastroenterology and Nutrition, University of Lisbon, Lisbon, Portugal

Correspondence to: Guida Maria Portela–Gomes, Dept. of Genetics and Pathology, Unit of Pathology, University Hospital, 75185 Uppsala, Sweden. E-mail: portela_gomes@yahoo.com

We studied the immunoreactivity of 12 different region-specific antibodies to the chromogranin A (CgA) molecule in the various neuroendocrine cell types of the human gastrointestinal (GI) tract by using double immunofluorescence techniques. These staining results were compared with others obtained with a commercial monoclonal CgA antibody (LK2H10). G (gastrin)-cells showed immunoreactivity to virtually all region-specific antibodies, but with varying frequency. Most intestinal EC (enterochromaffin)- and L (enteroglucagon)-cells were immunoreactive to the antibodies to the N-terminal and mid-portion of the CgA molecule, whereas the EC-cells in the stomach reacted with fewer region-specific antibodies. D (somatostatin)-cells reacted to the CgA 411–424 antibody and only occasionally showed immunoreactivity to the other CgA antibodies. A larger cytoplasmic area was stained with the antibodies to CgA 17–38 and 176–195 than with the other antibodies tested. These differences in staining pattern may reflect different cleavage of the CgA molecule in different cell types and at different regions of the GI tract.

(J Histochem Cytochem 50:1487–1492, 2002)

Key Words: chromogranin A, chromogranin A fragments, immunocytochemistry, gastrointestinal tract, human, neuroendocrine cells


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Nutr.Home page
M. Mazzoni, M. Le Gall, S. De Filippi, L. Minieri, P. Trevisi, J. Wolinski, G. Lalatta-Costerbosa, J.-P. Lalles, P. Guilloteau, and P. Bosi
Supplemental Sodium Butyrate Stimulates Different Gastric Cells in Weaned Pigs
J. Nutr., August 1, 2008; 138(8): 1426 - 1431.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
P. Bosi, M. Mazzoni, S. De Filippi, P. Trevisi, L. Casini, G. Petrosino, and G. Lalatta-Costerbosa
A Continuous Dietary Supply of Free Calcium Formate Negatively Affects the Parietal Cell Population and Gastric RNA Expression for H+/K+-ATPase in Weaning Pigs
J. Nutr., May 1, 2006; 136(5): 1229 - 1235.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
S. Wang, J. Liu, L. Li, and B. M. Wice
Individual Subtypes of Enteroendocrine Cells in the Mouse Small Intestine Exhibit Unique Patterns of Inositol 1,4,5-trisphosphate Receptor Expression
J. Histochem. Cytochem., January 1, 2004; 52(1): 53 - 64.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2002