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Journal of Histochemistry and Cytochemistry, Vol. 50, 1501-1508, November 2002, Copyright © 2002, The Histochemical Society, Inc.

ARTICLE

The Cellular Localization of Increased Atrial Natriuretic Peptide mRNA and Immunoreactivity in Diabetic Rat Kidneys

Feng J. Laia, Ming C. Hsiehb, Shih C. Hsinb, Shiu R. Linc, Jinn Y. Guhd, Hung C. Chend, and Shyi J. Shinb
a Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
b Division of Endocrinology and Metabolism, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
c Department of Clinical Pathology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
d Divisions of Nephrology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

Correspondence to: Shyi J. Shin, Div. of Endocrinology and Metabolism, Kaohsiung Medical University Hospital, 100 Shih-Chuan 1st Rd, Kaohsiung 80708, Taiwan. E-mail: d680032@kmu.edu.tw

Increased intrarenal atrial natriuretic peptide (ANP) mRNA expression has been reported in several disorders. To further investigate the action of renal ANP, we need to elucidate the exact site of its alteration in diseased kidneys. ANP mRNA and ANP were detected by in situ hybridization and immunohistochemistry in the kidneys from five normal and five diabetic rats. Renal ANP mRNA in eight normal and nine diabetic rats was measured by RT-PCR with Southern blot hybridization. In normal and diabetic rats, the distribution of ANP mRNA and ANP-like peptide was mainly located in proximal, distal, and collecting tubules. However, diabetic rats had significant enhancement of ANP mRNA and ANP-immunoreactive staining in the proximal straight tubules, medullary thick ascending limbs, and medullary collecting ducts. ANP mRNA in the outer and inner medulla of nine diabetic rats increased 5.5-fold and 3.5-fold, but only 1.8-fold in the renal cortex. This preliminary study showed that ANP mRNA and ANP immunoreactivity in proximal straight tubules, medullary thick ascending limb, and medullary collecting ducts apparently increased in diabetic kidneys. These findings imply that ANP synthesis in these nephrons may involve in adaptations of renal function in diabetes. (J Histochem Cytochem 50:1501–1507, 2002)

Key Words: atrial natriuretic peptide, proximal straight tubule, medullary thick ascending limb, collecting duct, distal convoluted tubule, diabetic kidney, angiotensin II, vasopressin


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