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Journal of Histochemistry and Cytochemistry, Vol. 50, 1641-1645, December 2002, Copyright © 2002, The Histochemical Society, Inc.

ARTICLE

Lac z Histochemistry and Immunohistochemistry Reveal Ephrin-B Ligand Expression in the Inner Ear

Lynne M. Bianchia, Kyl Dinsioa, Katherine Davolia, and Nicholas W. Galeb
a Neuroscience Program, Oberlin College, Oberlin, Ohio
b Regeneron Pharmaceuticals, Tarrytown, New York

Correspondence to: Lynne M. Bianchi, Neuroscience Program, Oberlin College, Oberlin, OH 44074. E-mail: lynne.bianchi@oberlin.edu

Immunostaining in transgenic mice carrying the lac z gene can be used to map gene and protein distribution in a single tissue. In this study, we examined inner ears from ephrin-B3 homozygous and ephrin-B2 heterozygous mice. Ephrin-B3 lac z expression was limited in these mice. However, immunostaining revealed ephrin-B3 throughout cochlear and vestibular regions. Immunoreactivity was absent in ephrin-B3-homozygous null mutants, demonstrating the specificity of the antibody. Ephrin-B2 lac z reactivity was detected in a limited number of cells in cochlear and vestibular regions. Different immunostaining patterns were found with different antibodies. Comparison with lac z expression indicated which antibody was specific for the transmembrane-bound ephrin-B2 ligand.

(J Histochem Cytochem 50:1641–1645, 2002)

Key Words: cochlea, vestibular, ephrin-B2, ephrin-B3, transgenic mice


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