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Journal of Histochemistry and Cytochemistry, Vol. 50, 311-324, March 2002, Copyright © 2002, The Histochemical Society, Inc.

ARTICLE

Heterogeneous Expression of Ca2+ Handling Proteins in Rabbit Sinoatrial Node

Hanny Musaa, Ming Leib, Hauro Honjoc, Sandra A. Jonesa, Halina Dobrzynskia, Mathew K. Lancastera, Yoshiko Takagishic, Zaineb Hendersona, Itsuo Kodamac, and Mark R. Boyetta
a School of Biomedical Sciences, University of Leeds, Leeds, United Kingdom
b University Laboratory of Physiology, University of Oxford, Oxford, United Kingdom
c Research Institute of Environmental Medicine, Nagoya University, Nagoya, Japan

Correspondence to: Mark R. Boyett, School of Biomedical Sciences, University of Leeds, Leeds LS2 9JT, UK. E-mail: m.r.boyett@leeds.ac.uk

We investigated the densities of the L-type Ca2+ current, iCa,L, and various Ca2+ handling proteins in rabbit sinoatrial (SA) node. The density of iCa,L, recorded with the whole-cell patch-clamp technique, varied widely in sinoatrial node cells. The density of iCa,L was significantly (p<0.001) correlated with cell capacitance (measure of cell size) and the density was greater in larger cells (likely to be from the periphery of the SA node) than in smaller cells (likely to be from the center of the SA node). Immunocytochemical labeling of the L-type Ca2+ channel, Na+-Ca2+ exchanger, sarcoplasmic reticulum Ca2+ release channel (RYR2), and sarcoplasmic reticulum Ca2+ pump (SERCA2) also varied widely in SA node cells. In all cases there was significantly (p<0.05) denser labeling of cells from the periphery of the SA node than of cells from the center. In contrast, immunocytochemical labeling of the Na+-K+ pump was similar in peripheral and central cells. We conclude that Ca2+ handling proteins are sparse and poorly organized in the center of the SA node (normally the leading pacemaker site), whereas they are more abundant in the periphery (at the border of the SA node with the surrounding atrial muscle). (J Histochem Cytochem 50:311–324, 2002)

Key Words: L-type Ca2+ channel, Na+-Ca2+ exchanger, ryanodine receptor, Ca2+ pump, sarcoplasmic reticulum


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