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Journal of Histochemistry and Cytochemistry, Vol. 50, 437-439, March 2002, Copyright © 2002, The Histochemical Society, Inc.

BRIEF REPORT

Simple Method for Reduction of Autofluorescence in Fluorescence Microscopy

Michael Neumanna and Detlef Gabela
a Department of Chemistry, University of Bremen, Bremen, Germany

Correspondence to: Detlef Gabel, Dept. of Chemistry, University of Bremen, Leobener Strasse, Postfach 330 440, D-28334 Bremen, Germany. E-mail: gabel@chemie.uni-bremen.de

Autofluorescence of aldehyde-fixed neural tissue often complicates the use of fluorescence microscopy. Background fluorescence can be notably reduced or eliminated by irradiation with light before treatment with fluorescence probes, resulting in a higher contrast without adversely affecting the staining probabilities. (J Histochem Cytochem 50:437–439, 2002)

Key Words: autofluorescence, photobleaching, immunofluorescence


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