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Journal of Histochemistry and Cytochemistry, Vol. 50, 1113-1130, August 2002, Copyright © 2002, The Histochemical Society, Inc.

ARTICLE

Localization of Laminin {alpha}4-Chain in Developing and Adult Human Tissues

Noora Petäjäniemia, Matti Korhonenb, Jarkko Kortesmaac,d, Karl Tryggvasonc, Kiyotoshi Sekiguchie, Hironobu Fujiwarae, Lydia Sorokinf, Lars-Eric Thornellg, Zenebech Wondimuh, Daniel Assefah, Manuel Patarroyoh, and Ismo Virtanena
a Institute of Biomedicine/Anatomy, Biomedicum Helsinki, University of Helsinki, Helsinki, Finland
b Hospital for Children and Adolescents, Helsinki University Central Hospital, Helsinki, Finland
c Division of Matrix Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
d BioStratum AB, Stockholm, Sweden
e Osaka University, Institute for Protein Research, Osaka, Japan
f IZKF Nachwuchsgruppe II, Nikolaus Fiebiger Center, Erlangen, Germany
g Department of Integrative Medical Biology, Anatomy, Umeå University, Umeå, Sweden
h Microbiology and Tumor Biology Center/Odontology, Karolinska Institute, Stockholm, Sweden

Correspondence to: Noora Petäjäniemi, Inst. of Biomedicine/Anatomy, Biomedicum Helsinki, PO Box 63 (Haartmaninkatu 8), FIN-00014 University of Helsinki, Helsinki, Finland. E-mail: noora.petajaniemi@helsinki.fi

Recent studies suggest important functions for laminin-8 (Ln-8; {alpha}4ß1{gamma}1) in vascular and blood cell biology, but its distribution in human tissues has remained elusive. We have raised a monoclonal antibody (MAb) FC10, and by enzyme-linked immunoassay (EIA) and Western blotting techniques we show that it recognizes the human Ln {alpha}4-chain. Immunoreactivity for the Ln {alpha}4-chain was localized in tissues of mesodermal origin, such as basement membranes (BMs) of endothelia, adipocytes, and skeletal, smooth, and cardiac muscle cells. In addition, the Ln {alpha}4-chain was found in regions of some epithelial BMs, including epidermis, salivary glands, pancreas, esophageal and gastric glands, intestinal crypts, and some renal medullary tubules. Developmental differences in the distribution of Ln {alpha}4-chain were detected in skeletal muscle, walls of vessels, and intestinal crypts. Ln {alpha}4- and Ln {alpha}2-chains co-localized in BMs of fetal skeletal muscle cells and in some epithelial BMs, e.g., in gastric glands and acini of pancreas. Cultured human pulmonary artery endothelial (HPAE) cells produced Ln {alpha}4-chain as Mr 180,000 and 200,000 doublet and rapidly deposited it to the growth substratum. In cell-free extracellular matrices of human kidney and lung, Ln {alpha}4-chain was found as Mr 180,000 protein. (J Histochem Cytochem 50:1113–1130, 2002)

Key Words: basement membrane, laminin-8, {alpha}4-chain, monoclonal antibody, endothelial cell


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