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Journal of Histochemistry and Cytochemistry, Vol. 50, 1195-1203, September 2002, Copyright © 2002, The Histochemical Society, Inc.

ARTICLE

CD9 Expression in Solid Non-neuroepithelial Tumors and Infiltrative Astrocytic Tumors

Masatou Kawashimaa,b, Katsumi Doh-uraa, Eisuke Mekadac, Masashi Fukuib, and Toru Iwakia
a Departments of Neuropathology
b Neurosurgery
c Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan, and Department of Cell Biology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan

Correspondence to: Masatou Kawashima, Dept. of Neurological Surgery, U. of Florida, PO Box 100265, Gainesville, FL 32610-0265. E-mail: kawasm@neurosurgery.ufl.edu

The tetraspan membrane protein CD9 is normally expressed in the mature myelin sheath and is believed to suppress the metastatic potential of certain human tumors. In this study we identified CD9 in a variety of brain tumors by immunohistochemical (IHC) and immunoblotting analyses. We examined 96 tumor samples and three glioma cell lines in addition to a murine brain tumor model of transplanted glioma cells in CD9-deficient mice and control mice. CD9 was expressed not only in solid non-neuroepithelial tumors but also in infiltrative malignant neuroepithelial tumors. Among the neuroepithelial tumors, high-grade astrocytic tumors, including glioblastomas and anaplastic astrocytomas, showed higher immunoreactivity than low-grade cerebral astrocytomas. Thus, CD9 expression in astrocytic tumors correlated with their malignancy. In the murine brain tumor model, transplanted glioma cells were shown to grow and spread through myelinated areas irrespective of the presence or absence of CD9 expression in the recipient's brain. These results indicate that the CD9 expression of astrocytic tumors plays a significant role in the malignancy independent of CD9 expression in the surrounding tissue. This might be explained by the observation that the CD9 molecule is associated with a mitogenic factor, membrane-anchored heparin-binding epidermal growth factor, which is known to be upregulated in malignant gliomas. (J Histochem Cytochem 50:1195–1203, 2002)

Key Words: brain tumors, CD9, astrocytic tumors, murine brain tumor model


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