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Journal of Histochemistry and Cytochemistry, Vol. 51, 121-124, January 2003, Copyright © 2003, The Histochemical Society, Inc.

BRIEF REPORT

Isolation of Bovine Retinal Pigment Epithelial Cells Using Adhesion to Agarose: Demonstration of Cellular and Regional Heterogeneity

Eleonore Fröhlicha, Elke Maiera, and Christian Klessena
a Anatomisches Institut der Eberhard-Karls-Universität, Tübingen, Germany

Correspondence to: Eleonore Fröhlich, Anatomisches Institut, Österbergstr. 3, 72074 Tübingen, Germany. E-mail: eleonore.froehlich@anatu.uni-tuebingen.de

The retinal pigment epithelium (RPE) shows cell heterogeneity in morphology and enzymatic activity. Routine isolation procedures for RPE cells may reduce enzymatic activity and prevent the quantification of regional enzymatic differences in vivo. We developed a new technique for the isolation of RPE cells based on adhesion of the cells to agarose. The morphology of the isolated cells resembled that of RPE cells in vivo. The cells were viable in the dye exclusion test and showed a histochemical staining pattern as RPE cells in vivo. With this technique, quantitative regional differences in the enzymatic activities were detected. (J Histochem Cytochem 51:121–124, 2003)

Key Words: retinal pigment epithelium, ultrastructure, enzymatic activity


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