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Journal of Histochemistry and Cytochemistry, Vol. 51, 31-39, January 2003, Copyright © 2003, The Histochemical Society, Inc.

ARTICLE

A New Method to Enhance Contrast of Ultrathin Cryosections for Immunoelectron Microscopy

Toshihiro Takizawaa, Clark L. Andersonb, and John M. Robinsona
a Departments of Physiology and Cell Biology, Ohio State University, Columbus, Ohio
b Internal Medicine, Ohio State University, Columbus, Ohio

Correspondence to: Toshihiro Takizawa, Dept. of Physiology and Cell Biology, Ohio State University, 302 Hamilton Hall, 1645 Neil Ave., Columbus, OH 43210. E-mail: takizawa.2@osu.edu

Adequate contrast of ultrathin cryosections is crucial for evaluating morphological detail to assess immunocytochemical localization at the electron microscopic level. We have developed a positive staining method for achieving contrast in ultrathin cryosections, from tissue fixed only in paraformaldehyde, that provides excellent contrast at the electron microscopic level. (J Histochem Cytochem 51:31–39, 2003)

Key Words: immunocytochemistry, placenta, caveolae, endothelium, trans-Golgi network


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