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Journal of Histochemistry and Cytochemistry, Vol. 51, 1255-1267, October 2003, Copyright © 2003, The Histochemical Society, Inc.

ARTICLE

Developmental Behavior of Embryonic Myogenic Progenitors Transplanted into Adult Muscle as Revealed by Desmin LacZ Recombinant Gene

Gwenola Auda–Bouchera, Thierry Rouauda, Josiane Fontaine–Pérusa, Fabien Le Granda, and Marie-France Gardahauta
a UMR CNRS 6018, Faculté des Sciences et des Techniques, Nantes, France

Correspondence to: Marie-France Gardahaut, UMR CNRS 6018, Faculté des Sciences et des Techniques, 2 rue de la Houssinière, 44322 Nantes Cedex, France. E-mail: Marie-France.Gardahaut@svt.univ-nantes.fr

We studied the behavior of myogenic progenitors from donor desmin+/- LacZ embryos after implantation into tibialis anterior muscle of 2-month-old mouse hosts. Myogenic progenitors were collected from 10-day post-coital mouse embryo somite dermomyotomes (DMs), forelimb buds (LBs), and trunks. The replacement of desmin by the LacZ coding sequence allowed specific monitoring of ß-galactosidase expression in donor myogenic cells. Immunostaining for myosin heavy chain and laminin expression was performed together with acetylcholine receptor histochemistry on sections of implanted muscle. Myogenic progenitors generated from DM, LB, and trunk were able to proliferate and adopt a myogenic pathway after transplantation into adult mouse muscle. Although their development appeared to be limited for DM and LB cell transplantation, the differentiation of myogenic progenitors occurred readily with trunk cell injection, suggesting that cell types associated with DM cells were involved in long-term myofiber differentiation (21 day). When neural tube/notochord (NTN) or sclerotomal (S) cells were co-transplanted with DM cells, myogenic nuclei were produced, indicating that both NTN and S are required for the differentiation of DMs grafted into adult muscle. These data are consistent with the differentiation of neural tissues and bone from NTN and S, respectively, and with the development of anatomic relations among all in vivo-differentiated tissues. These results suggest that embryonic trunk cells can be used to repair different types of injured tissues (especially skeletal muscle) under appropriate environmental conditions.

(J Histochem Cytochem 51:1255–1267, 2003)

Key Words: embryonic cells, muscle transplantation, desmin LacZ mouse, myogenic differentiation, neurogenic differentiation, chondrogenic differentiation


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