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Journal of Histochemistry and Cytochemistry, Vol. 51, 1299-1306, October 2003, Copyright © 2003, The Histochemical Society, Inc.

ARTICLE

Ultrastructural Orientation of Laminin 5 in the Epidermal Basement Membrane: an Updated Model for Basement Membrane Organization

James R. McMillana, Masashi Akiyamaa, and Hiroshi Shimizua
a Department of Dermatology, Hokkaido University Graduate School of Medicine, Sapporo, Japan

Correspondence to: James R. McMillan, Dept. of Dermatology, Hokkaido University Graduate School of Medicine, Kita-ku, Sapporo 060-8638, Japan. E-mail: jrm57@med.hokudai.ac.jp

Laminin 5 is a trimeric glycoprotein involved in cell adhesion in the epidermal basement membrane. To determine the precise orientation of laminin 5 in adult human skin, we used plural epitope-specific monoclonal antibodies, a polyclonal antiserum, and postembedding immunogold electron microscopy (IEM). Immunogold labeling distances from the basal keratinocyte plasma membrane (PM) were measured for each gold particle (>200 particles) and the mean distance (nm) calculated. Antibodies included BM165 (recognizing the {alpha}3-chain first globular domain) that was measured at 35.40 ± 2.20 nm from the keratinocyte PM, K140 (recognizing a region adjacent to the ß3-chain globular domain IV) that measured 45.20 ± 3.60 nm from the PM, and an anti-laminin 5 polyclonal antiserum that was 43.43 ± 6.28 nm from the PM. The laminin 5 {gamma}2-chain short arm hinge domain was previously localized to the lower lamina densa (LD) at approximately 56.30 ± 1.65 nm from the keratinocyte PM. Taken together with previous {gamma}2-chain data and the distribution of the polyclonal antisera, we estimate that the long axis of laminin 5 is oriented at an angle of approximately 27° from the horizontal lamina lucida (LL)/LD border and propose that the {gamma}2-chain lies farthest from the PM. This novel orientation, with the majority of the laminin 5 molecule lying obliquely along the LL/LD border and not perpendicularly, as was first thought, sheds new light on the organization of the basement membrane and likely molecular interactions. (J Histochem Cytochem 51:1299–1306, 2003)

Key Words: electron microscopy, hemidesmosome, extracellular matrix protein, laminin 6


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