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Journal of Histochemistry and Cytochemistry, Vol. 51, 1343-1353, October 2003, Copyright © 2003, The Histochemical Society, Inc.

ARTICLE

Chromosomal Variations Within Aneuploid Cancer Lines

Takahiro Isakaa, Andrea L. Nestora, Tadahiro Takadaa, and David C. Allisona
a Departments of Surgery, Physiology and Molecular Medicine, and the MCO Microscopy Imaging Center, Medical College of Ohio, Toledo, Ohio

Correspondence to: David C. Allison, Medical College of Ohio, 3035 Arlington Ave., Toledo, OH 43614-5804. E-mail: dallison@mco.edu

Aneuploid cancers exhibit a wide spectrum of clinical aggressiveness, possibly because of varying chromosome compositions. To test this, karyotypes from the diploid CCD-34Lu fibroblast and the aneuploid A549 and SUIT-2 cancer lines underwent fluorescence in situ hybridization (FISH) and DAPI counterstaining. The number of DAPI-stained and FISH-identified chromosomes, 1-22, X,Y, as well as structural abnormalities, were counted and compared using the {chi}2, Mann–Whitney rank sum test and the Levene's equality of variance. Virtually all of the evaluable diploid CCD-34Lu karyotypes had 46 chromosomes with two normal-appearing homologues. The aneuploid chromosome numbers per karyotype were highly variable, averaging 62 and 72 for the A549 and SUIT-2 lines, respectively. However, the A549 chromosome numbers were more narrowly distributed than the SUIT-2 karyotype chromosome numbers. Furthermore, 25% of the A549 chromosomes had structural abnormalities compared to only 7% of the SUIT-2 chromosomes. The chromosomal compositions of the aneuploid A549 and SUIT-2 cancer lines are widely divergent, suggesting that diverse genetic alterations, rather than chance, may govern the chromosome makeups of aneuploid cancers. (J Histochem Cytochem 51:1343–1353, 2003)

Key Words: aneuploidy, karyotypes, chromosomal abnormalities, cell lines, fluorescence in situ, hybridization


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