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Journal of Histochemistry and Cytochemistry
Volume 51 (12): 1601-1609, 2003
Copyright ©The Histochemical Society, Inc.

Identification of c-Jun as bcl-2 Transcription Factor in Human Uterine Endometrium

ZL Li, H. Abe, K. Ueki, K. Kumagai, R. Araki and Y. Otsuki

Departments of Anatomy and Biology (ZLL,HA,YO) and Obstetrics and Gynecology (KU,KK), Osaka Medical College, Osaka, Japan and Department of Obstetrics and Gynecology (RA), Takatsuki Red-Cross Hospital, Osaka, Japan

Correspondence to: Y. Otsuki, MD, Dept. of Anatomy and Biology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka 569-8686, Japan. E-mail: an1001{at}art.osaka-med.ac.jp

We describe the application of the biomolecular interaction (BIA) technique to detection of the interaction between protein (e.g., c-Jun) and DNA (e.g., two AP-1 motifs from bcl-2 promoter), compared with immunohistochemistry (IHC) of c-Jun. The specific binding assay for the interaction of c-Jun and activating protein-1 (AP-1) motifs was performed using a Biacore 2000 system. Intense immunoreactivity of c-Jun in glandular cells of the human uterine endometrium was observed in the proliferative phase, while c-Jun in stromal cells was expressed throughout the menstrual cycle. In contrast to the IHC of c-Jun, the specific binding of c-Jun to two separate AP-1 motifs in the bcl-2 promoter region was detected only in nuclear extracts of glandular cells, but not in stromal cells, during the proliferative phase. These results indicate that, while transmitting various signals, c-Jun enhances the transcription level of bcl-2, which in turn keeps glandular cells alive and proliferating in normal human endometrium during the proliferative phase. Moreover, the method involving real-time biomolecular interactions such as DNA–protein binding is novel for the study of transcription factors when combined with IHC.

(J Histochem Cytochem 51:1601–1609, 2003)

Key Words: biomolecular interaction • bcl-2 • c-Jun • AP-1 • promoter • endometrium • uterus


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