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Journal of Histochemistry and Cytochemistry, Vol. 51, 239-243, February 2003, Copyright © 2003, The Histochemical Society, Inc.

ARTICLE

Lectin Histochemistry Shows Fucosylated Glycoconjugates in the Primordial Germ Cells of Xenopus Embryos

Edurne Alonsoa, Francisco J. Sáeza, Juan F. Madridb, and Francisco Hernándezb
a Department of Cell Biology and Histology, University of the Basque Country, Leioa (Vizcaya), Spain
b Department of Cell Biology, University of Murcia, School of Medicine, Espinardo (Murcia), Spain

Correspondence to: Francisco J. Sáez, Universidad del País Vasco, Departamento de Biología Celular e Histología, Facultad de Medicina y Odontología, B&ogr; Sarriena s/n, E-48940 Leioa (Vizcaya), Spain. E-mail: gcpsacrf@lg.ehu.es

Previous works have shown that glycoconjugates with terminal fucose (Fuc) are located in the primordial germ cells (PGCs) of some mammals and might play a role in the migration and adhesion processes during development. The aim of this work was to identify the terminal Fuc moieties of Xenopus PGCs by means of three Fuc-binding lectins: from asparagus pea (LTA), gorse seed (UEA-I), and orange peel fungus (AAA). The histochemical procedures were also carried out after deglycosylation pretreatments: ß-elimination with NaOH to remove O-linked oligosaccharides; incubation with PNGase F to remove N-linked carbohydrate chains; and incubation with {alpha}(1,2)- and {alpha}(1,6)-fucosidase. The PGCs were always negative for LTA and UEA-I, two lectins that have the highest affinity for Fuc {alpha}(1,2)-linked. However, the PGCs were strongly labeled with AAA, which preferentially binds to Fuc with {alpha}(1,3) or {alpha}(1,4) linkages and to Fuc {alpha}(1,6)-linked to the proximal N-acetylglucosamine. There was fainter labeling with AAA when the sections were preincubated with {alpha}(1,6)-fucosidase, but the labeling remained strong when the sections were pretreated with {alpha}(1,2)fucosidase. When the ß-elimination procedure was carried out, the PGC labeling with AAA was slight. If the PNGase F incubation was performed, the PGCs remained moderately positive for AAA. These data suggest that the Xenopus PGCs have Fuc moieties in O- and N-linked oligosaccharides, including Fuc {alpha}(1,6) linked to the innermost GlcNAc, and that the Fuc was not in {alpha}(1,2)-linkage. (J Histochem Cytochem 51:239–243, 2003)

Key Words: N-linked oligosaccharides, O-linked oligosaccharides, fucosidase, ß-elimination, peptide N glycosidase F, Xenopus embryogenesis, germ cells


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