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Journal of Histochemistry and Cytochemistry, Vol. 51, 541-544, April 2003, Copyright © 2003, The Histochemical Society, Inc.

BRIEF REPORT

Intracellular Targeting of Calmodulin mRNAs in Primary Hippocampal Cells

Elod Kortvelya, Szilvia Varszegia, Arpad Palfia, and Karoly Gulyaa
a Department of Zoology and Cell Biology, University of Szeged, Szeged, Hungary

Correspondence to: Karoly Gulya, Dept. of Zoology and Cell Biology, University of Szeged, 2 Egyetem St., POB 659, Szeged H-6722, Hungary. E-mail: gulyak@bio.u-szeged.hu

We investigated the intracellular distribution of the mRNAs corresponding to the three non-allelic CaM genes in cultured hippocampal cells by in situ hybridization with digoxigenin-labeled gene-specific riboprobes. In neurons the perikaryon was heavily stained and strong dendritic mRNA targeting was detected for all three CaM genes. The color labeling exhibited a punctate distribution, suggesting that CaM mRNAs are transported in RNA granules. Immunocytochemistry for S100 demonstrated that glial cells express CaM mRNAs at a very low level. A minority of the cultured cells were negative for either labeling. (J Histochem Cytochem 51:541–544, 2003)

Key Words: calmodulin, mRNA localization, hippocampal cell culture, S100 immunocytochemistry, dendritic, intracellular


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