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Journal of Histochemistry and Cytochemistry, Vol. 51, 549-551, April 2003, Copyright © 2003, The Histochemical Society, Inc.

BRIEF REPORT

Fluorescence In Situ Hybridization (FISH) on Human Chromosomes Using Photoprobe Biotin-labeled Probes

Anja Weisea, Peter Harbarthb, Uwe Claussena, and Thomas Liehra
a Institute of Human Genetics, Jena, Germany
b Linaris, Wertheim–Bettingen, Germany

Correspondence to: Thomas Liehr, Institut für Humangenetik, Kollegiengasse 10, D-07743 Jena, Germany. E-mail: i8lith@mti.uni-jena.de

Fluorescence in situ hybridization (FISH) on human chromosomes in meta- and interphase is a well-established technique in clinical and tumor cytogenetics and for studies of evolution and interphase architecture. Many different protocols for labeling the DNA probes used for FISH have been published. Here we describe for the first time the successful use of Photoprobe biotin-labeled DNA probes in FISH experiments. Yeast artificial chromosome (YAC) and whole chromosome painting (wcp) probes were tested.

(J Histochem Cytochem 51:549–551, 2003)

Key Words: FISH, DNA labeling, Photoprobe system


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