Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yu, C.-M.
Right arrow Articles by Lan, H. Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yu, C.-M.
Right arrow Articles by Lan, H. Y.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 51, 625-631, May 2003, Copyright © 2003, The Histochemical Society, Inc.

ARTICLE

Expression of Macrophage Migration Inhibitory Factor in Acute Ischemic Myocardial Injury

Cheuk-Man Yua, Kevin Wing-Hon Laib, Yong-Xiong Chenb, Xiao-Ru Huangc, and Hui Y. Lanc
a Division of Cardiology, Department of Medicine & Therapeutics, The Chinese University of Hong Kong, Hong Kong
b Division of Cardiology, Department of Medicine, Queen Mary Hospital, University of Hong Kong, Hong Kong
c Division of Nephrology, Baylor College of Medicine, Houston, Texas

Correspondence to: Cheuk-Man Yu, Dept. of Medicine & Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong. E-mail: cmyu@cuhk.edu.hk

Macrophage migration inhibitory factor (MIF) is a key mediator in inflammatory or immune-mediated diseases, although its role in heart diseases is unknown. This study investigated the expression of MIF in the myocardium in the development of acute myocardial infarction (AMI). By use of immunohistochemistry, Western blotting, RT-PCR, and in situ hybridization, the gene and protein expression of MIF in the heart at 6 hr, 1 day, 3 days, 1 week, and 2 weeks after AMI was studied. In both normal and sham-operated rats, MIF mRNA and protein were expressed constitutively at low levels by the myocytes. By contrast, MIF mRNA was rapidly upregulated by the surviving myocytes in the infarcted region and, to a lesser extent, the non-infarcted region, accounting for a sevenfold increase at 6 hr after AMI (p<0.001). This was followed by a fourfold increase in MIF protein expression at day 1 after AMI (p<0.05). Macrophages were found accumulated in the infarcted region, being significant at day 1 (p<0.01) and progressive increased over the 2-week time course (p<0.01) in which MIF was found expressed in these cells. The results indicated that the infiltrating macrophages and myocytes were sources of MIF in the infarcted region. The latter cells became activated and involved in the amplification of inflammatory response in AMI. Therefore, upregulation of myocardial MIF may contribute to macrophage accumulation in the infarcted region and their pro-inflammatory role may participate in the myocyte damage seen in AMI. (J Histochem Cytochem 51:625–631, 2003)

Key Words: macrophage migration, inhibitory factor, macrophages, myocytes, myocardial infarction, gene expression


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 CirculationHome page
A. Zernecke, J. Bernhagen, and C. Weber
Macrophage Migration Inhibitory Factor in Cardiovascular Disease
Circulation, March 25, 2008; 117(12): 1594 - 1602.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
F. A. Amaral, C. T. Fagundes, R. Guabiraba, A. T. Vieira, A. L.S. Souza, R. C. Russo, M. P.B. Soares, M. M. Teixeira, and D. G. Souza
The Role of Macrophage Migration Inhibitory Factor in the Cascade of Events Leading to Reperfusion-Induced Inflammatory Injury and Lethality
Am. J. Pathol., December 1, 2007; 171(6): 1887 - 1893.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
M. J. Hardman, A. Waite, L. Zeef, M. Burow, T. Nakayama, and G. S. Ashcroft
Macrophage Migration Inhibitory Factor: A Central Regulator of Wound Healing
Am. J. Pathol., December 1, 2005; 167(6): 1561 - 1574.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
L. B. Garner, M. S. Willis, D. L. Carlson, J. M. DiMaio, M. D. White, D. J. White, G. A. Adams IV, J. W. Horton, and B. P. Giroir
Macrophage migration inhibitory factor is a cardiac-derived myocardial depressant factor
Am J Physiol Heart Circ Physiol, December 1, 2003; 285(6): H2500 - H2509.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2003