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Journal of Histochemistry and Cytochemistry, Vol. 51, 665-674, May 2003, Copyright © 2003, The Histochemical Society, Inc.

ARTICLE

Expression of MAL, an Integral Protein Component of the Machinery for Raft-mediated Apical Transport, in Human Epithelia

Mónica Marazuelaa, Agustín Acevedob, Magdalena Adradosb, María Angeles García–Lópeza, and Miguel A. Alonsoc
a Departmento de Endocrinología, Cantoblanco, Madrid, Spain
b Departamento de Patología, Cantoblanco, Madrid, Spain
c Hospital de la Princesa, and Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid and Consejo Superior de Investigaciones Científicas, Cantoblanco, Madrid, Spain

Correspondence to: Miguel A. Alonso, Centro de Biología Molecular Severo Ochoa, Facultad de Ciencias, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain. E-mail: maalonso@cbm.uam.es

The MAL protein is the only integral membrane protein identified as being an essential component of the machinery necessary for apical transport in the canine MDCK cell line, a paradigm of polarized epithelial cells. To characterize the range of human epithelia that use MAL-mediated pathways of transport, we performed an immunohistochemical survey of normal tissues using a monoclonal antibody (MAb) specific for the MAL protein. For comparison, different types of carcinoma were also analyzed. MAL, with a characteristic strong supranuclear granular distribution, was detected in specific types of normal epithelial cells throughout the respiratory system, the gastrointestinal and genitourinary tracts, and in exocrine and endocrine glands. Absorptive cells (e.g., enterocytes), and many different types of specialized secretory cells, either organized in discrete clusters (e.g., endocrine cells in the pancreas), gathered together in an endocrine gland (e.g., thyroid), interspersed with other cells in glands (e.g., parietal cells), or dispersed singly among other cells (e.g., type 2 pneumocytes) were positive for MAL. We also analyzed a series of epithelial renal and thyroid tumors and found alterations dependent on the particular histological type of tumor. These results open potential applications of the anti-MAL antibody for the characterization of neoplastic tissue. (J Histochem Cytochem 51:665–673, 2003)

Key Words: epithelial cell, sorting machinery, MAL protein, lipid rafts, carcinoma


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