Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by ten Dam, G. B.
Right arrow Articles by van Kuppevelt, T. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by ten Dam, G. B.
Right arrow Articles by van Kuppevelt, T. H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 51, 727-739, June 2003, Copyright © 2003, The Histochemical Society, Inc.

ARTICLE

Differential Expression of Heparan Sulfate Domains in Rat Spleen

Gerdy B. ten Dama, Theo Hafmansa, Jacques H. Veerkampa, and Toin H. van Kuppevelta
a Department of Biochemistry, Nijmegen Center for Molecular Life Sciences, University Medical Center Nijmegen, Nijmegen, The Netherlands

Correspondence to: Toin H. van Kuppevelt, Department of Biochemistry, Nijmegen Center for Molecular Life Sciences, University Medical Center Nijmegen 194, PO Box 9101, 6500 HB Nijmegen, The Netherlands. E-mail: a.vankuppevelt@ncmls.kun.nl

The microarchitecture of the spleen is composed of a meshwork of reticulum cells and their matrix. Heparan sulfates (HS) are important components of this meshwork and are involved in processes such as cell adhesion, cell migration, and cytokine/growth factor binding. The expression of HS epitopes was analyzed using anti-HS antibodies. Four different staining patterns were observed, as exemplified by antibodies RB4EA12, HS4E4, AO4B08, and HS4C3. These antibodies recognize different chemical modifications in HS. In adult spleen, RB4EA12 stained only the reticular meshwork and blood vessels in the red pulp and marginal zone. HS4E4 stained blood vessel-associated basal lamina. AO4B08 and HS4C3 stained the reticular meshwork and blood vessels throughout the spleen, but only AO4B08 strongly stained smooth muscle cells and ring fibers. Interleukin-2 localized in the red pulp and marginal zone and was bound to HS. AO4B08, HS4C3, and RB4EA12 but not HS4E4 co-localized with interleukin-2. In 10-day-old spleen, HS4E4 recognized reticular fibers, which were not stained in the adult stage. Immunoelectron microscopy revealed that HS was restricted to basal laminae and reticular fibers. Taken together, data show that HS epitopes are differentially expressed in the spleen and that this may create specific extracellular environments for immunological processes. (J Histochem Cytochem 51:727–739, 2003)

Key Words: glycosaminoglycans, heparan sulfates, extracellular matrix, single chain variable fragment, antibodies, lymphoid organs, spleen


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
R. R. Dinglasan, A. Alaganan, A. K. Ghosh, A. Saito, T. H. van Kuppevelt, and M. Jacobs-Lorena
Plasmodium falciparum ookinetes require mosquito midgut chondroitin sulfate proteoglycans for cell invasion
PNAS, October 2, 2007; 104(40): 15882 - 15887.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
G. B. ten Dam, E. M.A. van de Westerlo, A. Purushothaman, R. V. Stan, J. Bulten, F. C.G.J. Sweep, L. F. Massuger, K. Sugahara, and T. H. van Kuppevelt
Antibody GD3G7 Selected against Embryonic Glycosaminoglycans Defines Chondroitin Sulfate-E Domains Highly Up-Regulated in Ovarian Cancer and Involved in Vascular Endothelial Growth Factor Binding
Am. J. Pathol., October 1, 2007; 171(4): 1324 - 1333.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Kurup, T. J. M. Wijnhoven, G. J. Jenniskens, K. Kimata, H. Habuchi, J.-p. Li, U. Lindahl, T. H. van Kuppevelt, and D. Spillmann
Characterization of Anti-heparan Sulfate Phage Display Antibodies AO4B08 and HS4E4
J. Biol. Chem., July 20, 2007; 282(29): 21032 - 21042.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. B. t. Dam, S. Kurup, E. M. A. van de Westerlo, E. M. M. Versteeg, U. Lindahl, D. Spillmann, and T. H. van Kuppevelt
3-O-Sulfated Oligosaccharide Structures Are Recognized by Anti-heparan Sulfate Antibody HS4C3
J. Biol. Chem., February 24, 2006; 281(8): 4654 - 4662.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
P. Balogh, G. Horvath, and A. K. Szakal
Immunoarchitecture of Distinct Reticular Fibroblastic Domains in the White Pulp of Mouse Spleen
J. Histochem. Cytochem., October 1, 2004; 52(10): 1287 - 1298.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. B. ten Dam, E. M. A. van de Westerlo, T. F. C. M. Smetsers, M. Willemse, G. N. P. van Muijen, C. L. R. Merry, J. T. Gallagher, Y. S. Kim, and T. H. van Kuppevelt
Detection of 2-O-Sulfated Iduronate and N-Acetylglucosamine Units in Heparan Sulfate by an Antibody Selected against Acharan Sulfate (IdoA2S-GlcNAc)n
J. Biol. Chem., September 10, 2004; 279(37): 38346 - 38352.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2003