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Journal of Histochemistry and Cytochemistry, Vol. 51, 1017-1025, August 2003, Copyright © 2003, The Histochemical Society, Inc.

ARTICLE

Characterization of Matriptase Expression in Normal Human Tissues

Michael D. Obersta, Baljit Singha, Metin Ozdemirlia, Robert B. Dicksona, Michael D. Johnsona, and Chen-Yong Lina
a Lombardi Cancer Center, Georgetown University Medical Center, Washington, DC

Correspondence to: Chen-Yong Lin, Lombardi Cancer Center, New Research Building W412, Georgetown U. Medical Center, 3970 Reservoir Rd. NW, Box 571469, Washington, DC 20057-1421. E-mail: lincy@georgetown.edu

Matriptase is a type II transmembrane serine protease that has been implicated in the progression of epithelium-derived tumors. The role of this protease in the biology of normal epithelial cells remains to be elucidated. Matriptase mRNA has been detected by Northern analysis in tissues rich in epithelial cells, and the protein is expressed in vivo in normal and cancerous breast, ovarian, and colon tissues. However, a systematic analysis of the distribution of matriptase protein and mRNA in normal human tissues rich in epithelium has not been reported. In this study we characterized the expression of the protease in a wide variety of normal human tissues using a tissue microarray and whole tissue specimens. Significant immunoreactivity and mRNA expression were detected in the epithelial components of most epithelium-containing tissues. Matriptase expression was found in all types of epithelium, including columnar, pseudostratified columnar, cuboidal, and squamous. Distinct spatial distributions of reactivity were observed in the microanatomy of certain tissues, however. This suggests that although matriptase is broadly expressed among many types of epithelial cells, its activity within a tissue may be regulated in part at the protein and mRNA levels during the differentiation of selected epithelia.

(J Histochem Cytochem 51:1017–1025, 2003)

Key Words: matriptase, transmembrane serine, protease, hepatocyte growth factor, activator inhibitor-1 (HAI-1), immunohistochemistry


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