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Journal of Histochemistry and Cytochemistry, Vol. 51, 1105-1108, August 2003, Copyright © 2003, The Histochemical Society, Inc.

BRIEF REPORT

Simple and Rapid Methods for SEM Observation and TEM Immunolabeling of Rubber Particles

Adya P. Singha, Seung Gon Wib, Hunseung Kanga, Gap Chae Chunga, and Yoon Soo Kimb
a Agricultural Plant Stress Research Center, Chonnam National University, Gwangju, Republic of Korea
b Department of Forest Products and Technology, Chonnam National University, Gwangju, Republic of Korea

Correspondence to: Adya P. Singh, Agricultural Plant Stress Center, Chonnam National University, Gwangju 500-757, Republic of Korea. E-mail: adyasingh@hotmail.com

We developed a method involving air-drying of a rubber suspension after fixation in glutaraldehyde–tannic acid and postfixation in osmium tetroxide for SEM observation. For TEM immunolabeling the suspension was air-dried after osmium-only fixation. Whereas conventional methods failed to satisfactorily stabilize rubber particles, the methods described here proved successful in preserving their integrity.

(J Histochem Cytochem 51:1105–1108, 2003)

Key Words: rubber particles, air-drying, SEM, TEM immunolabeling


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