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Journal of Histochemistry and Cytochemistry, Vol. 51, 1237-1240, September 2003, Copyright © 2003, The Histochemical Society, Inc.

BRIEF REPORT

Ultrastructural Analysis of DNA Complexes During Transfection and Intracellular Transport

Régis Cartiera, Maria Velinovaa, Cathleen Lehmana, Bettina Erdmanna, and Regina Reszkaa
a Max-Delbrück Center for Molecular Medicine, Berlin, Germany

Correspondence to: Regina Reszka, AG Drug Targeting, Max-Delbrück Center for Molecular Medicine, Robert-Rössle-Str. 10, 12092 Berlin, Germany. E-mail: reszka@mdc-berlin.de

We present a simple method based on transmission electron microscopy that allows investigation of the early steps of polyplex-mediated transfection without the use of labeled DNA. The ultrastructural analysis showed internalization of 0.2–1-µm aggregates composed of 30–50-nm subunits. In addition, new details of the internalization process were revealed, suggesting an unspecific cell entry mechanism of large DNA aggregates.

(J Histochem Cytochem 51:1237–1240, 2003)

Key Words: gene transfer, polyplexes, transfection mechanism, NLS


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