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Journal of Histochemistry and Cytochemistry
Volume 52 (2): 187-192, 2004
Copyright ©The Histochemical Society, Inc.

Apoptosis in the Epididymal Epithelium of Adult Male Golden Hamster Exposed to Diethylstilbestrol

Chun-Hong Qiu, Masato Ohe, Noriyuki Koibuchi and Shigeru Matsuzaki

Department of Biochemistry, Dokkyo University School of Medicine (C-HQ,MO,SM), Tochigi, Japan, and Department of Integrative Physiology, Gunma University Graduate School of Medicine (C-HQ,NK), Gunma, Japan

Correspondence to: Chun-hong Qiu, Dept. of Integrative Physiology, Gunma Univ. Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan. E-mail: qiuchun{at}med.gunma-u.ac.jp

Apoptosis in the testis and prostate exposed to disrupters of endocrine function, including diethylstilbestrol (DES), during neonatal or postnatal periods has repeatedly been demonstrated, but not in the mature epididymis. We investigated the effects of DES, a potent and synthetic estrogen, on apoptosis in the adult. Adult male golden hamsters received an SC injection of DES and were then sacrificed to collect epididymides after 1, 4, or 7 days of treatment. A significant decrease in epididymal weight and an increase in apoptotic cells were shown on the first day after DES injection. Flow cytometry showed that DES treatment (1 mg/kg) for 1, 4, or 7 days induced significant apoptosis both in the caput and the cauda epididymides. Greater numbers of apoptotic cells were detected in the caput than in the cauda at a fixed time after DES treatment. Serum levels of testosterone decreased markedly within 24 hr after DES administration, reaching undetectable levels of 0.1 ng/ml at 4 days and thereafter. These results indicate that DES administration can increase epididymal apoptosis with a decrease in serum testosterone levels. Because DES used to be injected into domestic animals, adult males also have a chance to take this substance through food. Our study indicates that exposure to DES in adults is as toxic as that in the perinatal period. (J Histochem Cytochem 52:187–192, 2004)

Key Words: DES • epididymis • adult hamster • apoptosis • testosterone


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