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Volume 52 (4): 447-454, 2004
Copyright ©The Histochemical Society, Inc.
Distribution of Soluble Epoxide Hydrolase and of Cytochrome P450 2C8, 2C9, and 2J2 in Human Tissues
Departments of Pharmaceutical Sciences (AEE,MST,DFG) and Pathobiology and Veterinary Science (RAF,MST), University of Connecticut, Storrs, Connecticut
Correspondence to: David F. Grant, 372 Fairfield Road, HGH390 U-92, Storrs, CT 06269. E-mail: david.grant{at}uconn.edu
Soluble epoxide hydrolase (sEH) hydrolyzes a wide variety of endogenous and exogenous epoxides. Many of these epoxides are believed to be formed by cytochrome P450 epoxygenases. Here we report the distribution of sEH and cytochrome P450 epoxygenases 2C8, 2C9, and 2J2 by immunohistochemistry. A large number of different tissues from different organs were evaluated using high-throughput tissue microarrays. sEH was found in the liver, kidney, and in many other organs, including adrenals, pancreatic islets, pituitary gland, lymphoid tissues, muscles, certain vascular smooth muscles, and epithelial cells in the skin, prostatic ducts, and the gastrointestinal tract. Immunolabeling for sEH was highly specific for particular tissues and individual cell types. CYP2C9 was also found in almost all of these organs and tissues, suggesting that 2C9 and sEH are very similar in their tissue-specific patterns of expression. CYP2C8 and 2J2 were also widely distributed in human tissues but were less frequently associated with sEH. The results suggest potentially distinct pathways of endogenous fatty acid epoxide production and hydrolysis in a variety of human tissues. (J Histochem Cytochem 52:447–454, 2004)
Key Words: soluble epoxide hydrolase • cytochrome P450 • human tissues • immunohistochemistry
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