Search:        >> Advanced Search
Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kameda, Y. Articles by Chisaka, O. Search for Related Content PubMed PubMed Citation Articles by Kameda, Y. Articles by Chisaka, O. Social Bookmarking                      What's this?

The Role of Hoxa3 Gene in Parathyroid Gland Organogenesis of the Mouse

Yoko Kameda, Yuta Arai, Toshiyuki Nishimaki and Osamu Chisaka

Department of Anatomy, Kitasato University School of Medicine (YK,YA,TN), Sagamihara, Kanagawa, and Department of Cell and Developmental Biology, Graduate School of Biostudies, Kyoto University (OC), Kyoto, Japan

Correspondence to: Yoko Kameda, Dept. of Anatomy, Kitasato University School of Medicine, Sagamihara, Kanagawa 228-8555, Japan. E-mail: kameda{at}med.kitasato-u.ac.jp

Mice with a targeted deletion of the Hoxa3 gene have defects of derivatives of the third branchial arch and pouch. To address the role of the Hoxa3 gene in parathyroid organogenesis, we examined the third pharyngeal pouch development by immunohistochemistry (IHC) using the secretory protein (SP)-1/chromogranin A antiserum, which recognizes the parathyroid from its initial formation onward. At embryonic day (E) 11.5, the SP-1/chromogranin A-immunoreactive primary rudiment of the parathyroid appeared in the cranial region of the third pharyngeal pouch of wild-type embryos. In Hoxa3-null mutants, the third pharyngeal pouch was normally formed but failed to differentiate into the parathyroid rudiment, showing no immmunoreactivity for SP-1/chromogranin A. Classic studies using chick–quail chimeras have demonstrated that the ectomesenchymal neural crest cells are required for proper development of the pharyngeal pouch-derived organs, including the thymus and parathyroid glands. To visualize the migration and development of mesenchymal neural crest cells in Hoxa3 mutants, the heterozygotes were crossed with connexin43–lacZ transgenic mice in which ß-galactosidase expression was specific to the neural crest cells. In Hoxa3 homozygotes and in wild types, ectomesenchymal neural crest cells densely populated the pharyngeal arches, including the third one, and surrounded the third pouch epithelium. These results indicate that lack of the Hoxa3 gene affects the intrinsic ability of the third pharyngeal pouch to form the parathyroid rudiment and has no detectable effect on the migration of neural crest cells. (J Histochem Cytochem 52:641–651, 2004)

Key Words: Hoxa3 • parathyroid • thymus • third pharyngeal pouch • SP-1/chromogranin A • connexin43–lacZ transgenic • mice • neural crest cells • apoptosis

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				Y. Kameda, T. Nishimaki, O. Chisaka, S. Iseki, and H. M. Sucov Expression of the Epithelial Marker E-Cadherin by Thyroid C Cells and Their Precursors During Murine Development J. Histochem. Cytochem., October 1, 2007; 55(10): 1075 - 1088. [Abstract] [Full Text] [PDF]

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2004