Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
DOI: 10.1369/jhc.4A6273.2004
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Luo, H.
Right arrow Articles by Liu, Z.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Luo, H.
Right arrow Articles by Liu, Z.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry
Volume 52 (6): 797-803, 2004
Copyright ©The Histochemical Society, Inc.

Immunohistochemical Detection of Islet-1 and Neuronal Nitric Oxide Synthase in the Dorsal Root Ganglia (DRG) of Sheep Fetuses During Gestation

Haoshu Luo, Sheng Cui, Dawei Chen, Jiali Liu and Zhongxia Liu

Department of Animal Physiology, College of Biological Sciences (HL,SC,DC,JL,ZL) and Faculty of Veterinary Medicine (SC), China Agricultural University, Beijing, People's Republic of China

Correspondence to: Dr. S. Cui, Dept. of Animal Physiology, College of Biological Sciences, China Agricultural University, Beijing 100094, People's Republic of China. E-mail: cuisheng{at}cau.edu.cn

This study first investigated the ontogeny of Islet-1 and neuronal nitric oxide synthase (nNOS) expression and their co-localization in the DRG of sheep fetuses during gestation by immunohistochemistry (IHC). The results showed that Islet-1 and nNOS were located in the nuclei and cytoplasm of DRG neurons, respectively. The relative percentages of Islet-1-immunopositive (Islet-1+) neurons accounting for the total DRG neurons were 90%, 79%, 66%, and 53% at days 60, 90, and 120 of gestation and postnatally, respectively. The percentage of nNOS-immunopositive (nNOS+) neurons was 94% at day 60 and declined to ~30% at day 90, with no obvious further change until the postnatal period. Dual IHC showed that ~69% Islet-1+ neurons express nNOS at day 60 of gestation. This proportion declined to ~24% at day 90, after which there was no significant change until birth. We also observed that most Islet-1+ and nNOS+ neurons belonged to small and medium-sized DRG neurons from day 90 of gestation to the postnatal period. These results suggest that both Islet-1 and nNOS are important for the differentiation and maintenance of some specific phenotypes of DRG neurons during late gestation of sheep fetuses, although the related mechanisms need to be further elucidated.

(J Histochem Cytochem 52:797–803, 2004)

Key Words: Islet-1 • nNOS • DRG • immunohistochemistry • sheep fetuses


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2004