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DOI: 10.1369/jhc.4B6304.2004
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Journal of Histochemistry and Cytochemistry
Volume 52 (9): 1245-1248, 2004
Copyright ©The Histochemical Society, Inc.

BRIEF REPORT

Co-localization of Trax and Mea2 in Golgi Complex of Pachytene Spermatocytes in the Mouse

Miyuki Matsuda, Masaaki Kondo, Shin-ichi Kashiwabara, Mitsuyo Yoshihara, Shizuyo Sutou and Shoichi Matsukuma

Kanagawa Cancer Center Research Institute, Yokohama (MM,MY,SM); Ito Life Sciences Inc., Ibaraki (MK); Institute of Applied Biochemistry, University of Tsukuba, Tsukuba (SK); and School of Pharmacy, Shujitsu University (SS), Okayama, Japan

Correspondence to: Shoichi Matsukuma, PhD, Kanagawa Cancer Center Research Institute, Nakao 1-1-2, Asahi-ku, Yokohama 241-0815, Japan. E-mail: matsukum{at}gancen.asahi.yokohama.jp

A golgin family protein, Mea2, is expressed at enhanced level in pachytene spermatocytes and is indispensable for mouse spermatogenesis. Because Trax was shown to interact with Mea2 in yeast two-hybrid, we investigated the localization of Trax in pachytene spermatocytes with immunofluorescent staining. Trax was found to accumulate in the Golgi complex of mid-late pachytene spermatocytes and intermingled with granular Mea2 signal in the central region. In a subline of the Mea2 mutant mouse, a truncated form of Mea2 devoid of the N-terminal region, {Delta}Mea2, was expressed. It localized to the rim of Golgi complex and thus occupied a region separate from that of Trax.

(J Histochem Cytochem 52:1245–1248, 2004)

Key Words: Golgi complex • spermatocytes • mutant mouse • golgin-160


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