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Originally published as JHC exPRESS on August 22, 2005.
doi:10.1369/jhc.5A6645.2005
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Journal of Histochemistry and Cytochemistry
Volume 54 (1): 63-73, 2006
Copyright ©The Histochemical Society, Inc.

Expression of Serum Amyloid A, in Normal, Dysplastic, and Neoplastic Human Colonic Mucosa: Implication for a Role in Colonic Tumorigenesis

Orit Gutfeld1, Diana Prus1, Zvi Ackerman, Shira Dishon, Reinhold P. Linke, Mark Levin and Simcha Urieli-Shoval

Hematology Unit (SU-S,SD,ML), Departments of Oncology (OG), Pathology (DP), and Internal Medicine (ZA), Hadassah-Hebrew University Medical Center, Mount Scopus and Ein-Kerem, Jerusalem, Israel, and the Max-Planck-Institut für Biochemie, Martinsried, Germany (RPL)

Correspondence to: Simcha Urieli-Shoval, Hematology Unit, Hadassah-Hebrew University Medical Center, Mount Scopus, Jerusalem 91240, Israel. E-mail: simcha{at}hadassah.org.il

Serum amyloid A (SAA) is an acute phase reactant, whose level in the blood is elevated in response to trauma, infection, inflammation, and neoplasia. Elevated levels of SAA in the serum of cancer patients were suggested to be of liver origin rather than a tumor cell product. The role of SAA in human malignancies has not been elucidated. We investigated the expression of SAA at various stages of human colon carcinoma progression. Nonradioactive in situ hybridization applied on paraffin tissue sections from 26 colon cancer patients revealed barely detected SAA mRNA expression in normal looking colonic epithelium. Expression was increased gradually as epithelial cells progressed through dysplasia to neoplasia. Deeply invading colon carcinoma cells showed the highest levels of SAA. Expression was also found in colon carcinoma metastases. Cells of lymphoid follicles of the intestinal wall, inflammatory cells, ganglion cells, and endothelial cells, also expressed SAA mRNA. Immunohistochemical staining revealed SAA protein expression that colocalized with SAA mRNA expression. RT-PCR analysis confirmed the expression of the SAA1 and SAA4 genes in colon carcinomas, expression that was barely detectable in normal colon tissues. These findings indicate local and differential expression of SAA in human colon cancer tissues and suggest its role in colonic tumorigenesis. (J Histochem Cytochem 54:63–73, 2006)

Key Words: serum amyloid A • colon tissues • colonic neoplasia


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