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Originally published as JHC exPRESS on July 24, 2006.
doi:10.1369/jhc.5A6880.2006
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Journal of Histochemistry and Cytochemistry
Volume 54 (11): 1255-1261, 2006
Copyright ©The Histochemical Society, Inc.

Cellular Prion Protein in the Bovine Mammary Gland Is Selectively Expressed in Active Lactocytes

Andrea Didier, Richard Dietrich, Martin Steffl, Manfred Gareis, Martin H. Groschup, Simone Müller-Hellwig, Erwin Märtlbauer and Werner M. Amselgruber

Institute for Hygiene and Technology of Milk, Ludwig-Maximilian Universität, Munich, Germany (AD,RD,EM); Anatomy and Physiology of Domestic Animals, University of Hohenheim, Stuttgart, Germany (MS,WMA); Institute for Microbiology and Toxicology, Federal Research Centre for Nutrition and Food, Kulmbach, Germany (MG); Federal Research Institute for Animal Health, Friedrich-Loeffler-Institute, Insel Riems, Germany (MHG); and Unit of Microbiology, Zentralinstitut für Ernährungs- und Lebensmittelforschung, Technische Universität München, Freising, Germany (SM-H)

Correspondence to: Prof. W. Amselgruber, Anatomy and Physiology of Domestic Animals, University of Hohenheim, Fruwirthstraße 35 70599, Stuttgart, Germany. E-mail: amselgru{at}uni-hohenheim.de

The cellular prion protein (PrPc) is a highly conserved glycoprotein with a still enigmatic physiological function. It is mainly expressed in the central nervous system but accumulating data suggest that PrPc is also found in a broad spectrum of non-neuronal tissue. Here we investigated the cell-type-related PrPc expression in the bovine mammary gland by using immunohistochemistry (IHC), ELISA, Western blot, and real-time RT-PCR. Specific immunostaining of serial sections revealed that PrPc is selectively localized in mammary gland epithelial cells. Particularly strong expression was found at the basolateral surface of those cells showing active secretion. Results obtained by RT-PCR and ELISA complemented IHC findings. No correlation was found between the level of PrPc expression and other parameters such as age of the animals under study or stage of lactation. J Histochem Cytochem 54:1255–1261, 2006)

Key Words: immunohistochemistry • mammary gland • mRNA • prion protein • PrPc expression


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