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Originally published as JHC exPRESS on May 30, 2006.
doi:10.1369/jhc.6P6945.2006
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Journal of Histochemistry and Cytochemistry
Volume 54 (9): 961-964, 2006
Copyright ©The Histochemical Society, Inc.

PERSPECTIVES

Heat-induced Antigen Retrieval: What Are We Retrieving?

Thomas Boenisch

DakoCytomation, Carpinteria, California

Correspondence to: Thomas Boenisch, DakoCytomation R/D, 6392 Via Real, Carpinteria, CA 93013. E-mail: tom.boenisch{at}dakocytomation.com

Summary

Of all molecular aspects lost and recovered during formalin fixation and antigen retrieval, respectively, electrostatic charges have probably received the least attention. This review will focus on our work during the past 7 years. It strongly supports the tenet that electrostatic charges, i.e., net negative on antigens and net positive on antibodies, play an important part in immune reactions and therefore should be given greater attention when focusing on quality control in immunohistochemistry. (J Histochem Cytochem 54:961–964, 2006)

Key Words: antigen retrieval • epitope unmasking • immunohisotochemistry


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