This Article Full Text Full Text (PDF) All Versions of this Article: jhc.7A7207.2007v1 55/10/1039    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schmalzigaug, R. Articles by Premont, R. T. Search for Related Content PubMed PubMed Citation Articles by Schmalzigaug, R. Articles by Premont, R. T. Social Bookmarking                      What's this?

Differential Expression of the ARF GAP Genes GIT1 and GIT2 in Mouse Tissues

Robert Schmalzigaug, Hyewon Phee, Collin E. Davidson, Arthur Weiss and Richard T. Premont

Department of Medicine, Duke University Medical Center, Durham, North Carolina (RS,CED,RTP), and Department of Medicine, Howard Hughes Medical Institute, University of California San Francisco, San Francisco, California (HP,AW)

Correspondence to: Richard T. Premont, Department of Medicine, Duke University Medical Center, Box 3083, Durham, NC 27710. E-mail: richard.premont{at}duke.edu

GIT1 and GIT2 belong to the family of ADP-ribosylation factor GTPase-activating proteins (ARF-GAP) and have been implicated in the regulation of G protein-coupled receptor sequestration, cell migration, T-cell activation, neuronal spine formation, and aggregate formation in Huntington's disease. Examination of endogenous GIT protein expression in tissues, however, has been hampered by the lack of GIT2-specific antibodies. To visualize GIT1 and GIT2 gene expression in mouse tissues, we created mice with ß-galactosidase (ß-Gal) reporters inserted into the two GIT genes. ß-Gal staining confirmed the broad tissue distribution of GIT1 and GIT2 in the mouse but also revealed striking differences. GIT2 is expressed in most cells of the body, whereas GIT1 is restricted to only a subset of cells. For example, GIT2 is uniformly expressed throughout lung and liver, whereas GIT1 is restricted to cells lining blood vessels, bronchi, and bile ducts. Expression of GIT1 and GIT2 is mutually exclusive in the testes, where a developmental expression shift occurs, with GIT2 present in spermatogonia but GIT1 in mature spermatids. In conclusion, analysis of endogenous GIT expression revealed a nearly ubiquitous distribution of GIT2, whereas GIT1 is restricted to specific cell types even in tissues with apparently high GIT1 expression and is entirely absent from some tissues. (J Histochem Cytochem 55:1039–1048, 2007)

Key Words: GIT1 • GIT2 • ADP-ribosylation factor • GTPase-activating proteins • gene expression • ß-galactosidase • mice

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				R. A. Kahn, E. Bruford, H. Inoue, J. M. Logsdon Jr., Z. Nie, R. T. Premont, P. A. Randazzo, M. Satake, A. B. Theibert, M. L. Zapp, et al. Consensus nomenclature for the human ArfGAP domain-containing proteins J. Cell Biol., September 22, 2008; 182(6): 1039 - 1044. [Abstract] [Full Text] [PDF]

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2007